Kdc2, a Functional Homomeric Potassium Channel Expressed During Carrot Embryogenesis.
From: Department of Biology, University of Padova, via U. Bassi 58/B, 35131 Padova, Italy. elide@cribi.unipd.it
FEBS letters
- Publish Date: Sep 2006
- ISSN: 0014-5793
- Volume: 580
- Issue: 21
- Pages: 5009-15
- Medium: Print
- Language: English
- Citation (JAMA): Formentin Elide, Naso Alessia, Varotto Serena, et al. Kdc2, a Functional Homomeric Potassium Channel Expressed During Carrot Embryogenesis.. FEBS Lett. Sep 2006;580:5009-15
Abstract
In Daucus carota, the model system for embryogenesis, it has been demonstrated that potassium and K(+) selective channels are involved in embryo development. Here, we report the isolation and cloning of a new carrot Shaker-like potassium channel, potassium D. carota channel 2 (KDC2), whose expression pattern during somatic embryogenesis proceeds along with the establishment of the polar axes and the settlement of the hypocotyl region. In plants, KDC2 transcript is localized at the shoot level, in the epidermis and guard cells, similarly to its Arabidopsis homolog KAT1. Electrophysiological assays indicated KDC2 as the first carrot subunit able to form homomeric functional channels in Xenopus oocytes, with properties similar to those of Arabidopsis KAT1.
Mesh Headings (Keywords): Amino Acid Sequence, Animals, Cloning, Molecular, DNA, Complementary, Daucus carota, Embryonic Development, Gene Expression Profiling, Gene Expression Regulation, Plant, Molecular Sequence Data, Oocytes, Phylogeny, Plant Proteins, Potassium Channels, RNA, Messenger, Seedling, Seeds, Sequence Alignment, Sequence Analysis, DNA, Sequence Analysis, Protein, Xenopus
Check for Full Text / PubMed Unique Identifier (PMID): 16934807
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