Authentication of the Botanical and Geographical Origin of Honey by Front-face Fluorescence Spectroscopy.
From: Swiss Bee Research Centre/Agroscope Liebefeld-Posieux, Schwarzenburgstrasse 161, CH-3003 Berne, Switzerland. kaspar.ruoff@inframiel.ch
Journal of agricultural and food chemistry
- Publish Date: Sep 2006
- ISSN: 0021-8561
- Volume: 54
- Issue: 18
- Pages: 6858-66
- Medium: Print
- Language: English
- Citation (JAMA): Ruoff Kaspar, Luginbühl Werner, Künzli Raphael, et al. Authentication of the Botanical and Geographical Origin of Honey by Front-face Fluorescence Spectroscopy.. J. Agric. Food Chem. Sep 2006;54:6858-66
Abstract
Front-face fluorescence spectroscopy, directly applied on honey samples, was used for the authentication of 11 unifloral and polyfloral honey types (n = 371 samples) previously classified using traditional methods such as chemical, pollen, and sensory analysis. Excitation spectra (220-400 nm) were recorded with the emission measured at 420 nm. In addition, emission spectra were recorded between 290 and 500 nm (excitation at 270 nm) as well as between 330 and 550 nm (excitation at 310 nm). A total of four different spectral data sets were considered for data analysis. Chemometric evaluation of the spectra included principal component analysis and linear discriminant analysis; the error rates of the discriminant models were calculated by using Bayes’ theorem. They ranged from <0.1% (polyfloral and chestnut honeys) to 9.9% (fir honeydew honey) by using single spectral data sets and from <0.1% (metcalfa honeydew, polyfloral, and chestnut honeys) to 7.5% (lime honey) by combining two data sets. This study indicates that front-face fluorescence spectroscopy is a promising technique for the authentication of the botanical origin of honey and may also be useful for the determination of the geographical origin within the same unifloral honey type.
Mesh Headings (Keywords): Animals, Bees, Flowers, Food Contamination, Honey, Reproducibility of Results, Spectrometry, Fluorescence
Check for Full Text / PubMed Unique Identifier (PMID): 16939350
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.