Multiple Features Contribute to the Use of the Immunoglobulin M Secretion-specific Poly(A) Signal but Are Not Required for Developmental Regulation.
From: Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky, 800 Rose St., 108A Combs Building, Lexington, KY 40536-0096, USA. mlpete01@uky.edu
Molecular and cellular biology
- Publish Date: Sep 2006
- ISSN: 0270-7306
- Volume: 26
- Issue: 18
- Pages: 6762-71
- Medium: Print
- Language: English
- Citation (JAMA): Peterson Martha L, Bingham Gina L, Cowan Clarissa, et al. Multiple Features Contribute to the Use of the Immunoglobulin M Secretion-specific Poly(A) Signal but Are Not Required for Developmental Regulation.. Mol. Cell. Biol. Sep 2006;26:6762-71
Abstract
The secretory-specific poly(A) signal (mus) of the immunoglobulin mu gene plays a central role in regulating alternative RNA processing to produce RNAs that encode membrane-associated and secreted immunoglobulins. This poly(A) signal is in direct competition with a splice reaction, and regulation requires that these two reaction efficiencies be balanced. The mus poly(A) signal has several unique sequence features that may contribute to its strength and regulation. Site-directed mutations and small internal deletions made in the intact mu gene show that an extensive AU/A-rich sequence surrounding AAUAAA enhances signal use and that, of the two potential downstream GU-rich elements, both of which appear suboptimally located, only the proximal GU-rich sequence contributes substantially to use of this signal. A GU-rich sequence placed at a more standard location did not improve mus poly(A) signal use. All mu genes tested that contained modified mus poly(A) signals were developmentally regulated, indicating that the GU-rich sequences, the sequences between them previously identified as suboptimal U1A binding sites, and an upstream suboptimal U1A site do not contribute to mu mRNA processing regulation. Expression of wild-type and modified mu genes in HeLa cells overexpressing U1A also failed to demonstrate that U1A contributes to mus poly(A) signal regulation.
Mesh Headings (Keywords): Animals, Base Sequence, DNA-Directed RNA Polymerases, Gene Expression Regulation, Developmental, Hela Cells, Humans, Immunoglobulin M, Mice, Molecular Sequence Data, Octamer Transcription Factors, Plasma Cells, Poly A, RNA 3’ Polyadenylation Signals, RNA-Binding Proteins, Ribonucleoprotein, U1 Small Nuclear, Sequence Deletion
Check for Full Text / PubMed Unique Identifier (PMID): 16943419
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