Participation of Proteasome-associating Complex Pc500 in Starfish Oocyte Maturation As Revealed by Monoclonal Antibodies.
From: Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial Science and Technology (AIST), Toyohira-ku, Sapporo 062-8517, Japan. takagi.sawadamichiko@aist.go.jp
Biochemical and biophysical research communications
- Publish Date: Oct 2006
- ISSN: 0006-291X
- Volume: 349
- Issue: 2
- Pages: 694-700
- Medium: Print
- Language: English
- Citation (JAMA): Sawada Michiko Takagi, Tamura Tomohiro, Mitani Yasuo, et al. Participation of Proteasome-associating Complex Pc500 in Starfish Oocyte Maturation As Revealed by Monoclonal Antibodies.. Biochem. Biophys. Res. Commun. Oct 2006;349:694-700
Abstract
We previously reported that immature starfish oocytes contain a novel 530-kDa proteasome-associating complex PC500 [previously named PC530; E. Tanaka, M. Takagi Sawada, C. Morinaga, H. Yokosawa, H. Sawada, Isolation and characterization of a novel 530-kDa protein complex (PC 530) capable of associating with the 20S proteasome from star fish oocytes, Arch. Biochem. Biophys. 374 (2000) 181-188]. In the present study, in order to obtain an insight into the biological function of this complex, we investigated the effects of anti-PC500 monoclonal antibodies on oocyte maturation of the starfish Asterina pectinifera. A monoclonal antibody 7C5 strongly inhibited germinal vesicle breakdown (GVBD) in a concentration-dependent manner. In contrast to the inhibitory effect of the 7C5 antibody on GVBD, no inhibition of egg cleavage was observed in a 7C5-antibody-microinjected single blastomere in a 2-cell stage embryo. These results indicate that PC500 plays a key role in starfish oocyte maturation in a meiosis-specific manner.
Mesh Headings (Keywords): Animals, Antibodies, Monoclonal, Electrophoresis, Gel, Two-Dimensional, Mice, Mice, Inbred BALB C, Mitochondria, Multiprotein Complexes, Oocytes, Proteasome Endopeptidase Complex, Starfish, Ubiquitin
Check for Full Text / PubMed Unique Identifier (PMID): 16949555
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