Inhibition of Human Tumor Xenograft Growth in Nude Mice by a Conjugate of Monoclonal Antibody La22 to Epidermal Growth Factor Receptor with Anti-tumor Antibiotics Mitomycin C.
From: Welson Pharmaceuticals, Inc., 10239 Threadneedle CT, Ellicott City, MD 21042, USA.
Biochemical and biophysical research communications
- Publish Date: Oct 2006
- ISSN: 0006-291X
- Volume: 349
- Issue: 2
- Pages: 816-24
- Medium: Print
- Language: English
- Citation (JAMA): Shao Wei, Zhao Shan, Liu Zhaofei, et al. Inhibition of Human Tumor Xenograft Growth in Nude Mice by a Conjugate of Monoclonal Antibody La22 to Epidermal Growth Factor Receptor with Anti-tumor Antibiotics Mitomycin C.. Biochem. Biophys. Res. Commun. Oct 2006;349:816-24
Abstract
Anti-EGFR monoclonal antibodies LA22 and Erbitux bind to different epitopes of EGFR. The chemimmunoconjugates of MMC with LA22 or Erbitux were prepared, and in vitro cytotoxicity assays with A549 cells showed that LA22-MMC was much more potent than Erbitux or Erbitux-MMC. Viabilities of A549 cells treated with LA22-MMC, Erbitux or Erbitux-MMC were 35%, 94%, and 81%, respectively. Immunoscintigraphy of xenografts of human A431 and A549 cells in nude mice both showed that (125)I-labeled-LA22-MMC enriched in tumor sites prominently. Most importantly, in vivo assays showed LA22-MMC was significantly more effective than free drug MMC in the treatment of subcutaneous xenografts of human A431 cells in nude mice (83% inhibition for LA22-MMC and 30% for MMC). We concluded that LA22-MMC could be a very potent drug for treatment of solid tumors.
Mesh Headings (Keywords): Animals, Antibodies, Monoclonal, Antineoplastic Agents, Cell Line, Tumor, Female, Humans, Hybridomas, Mice, Mice, Inbred BALB C, Mice, Nude, Mitomycin, Neoplasm Transplantation, Receptor, Epidermal Growth Factor
Check for Full Text / PubMed Unique Identifier (PMID): 16950201
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