The B' Protein Phosphatase 2a Regulatory Subunit Well-rounded Regulates Synaptic Growth and Cytoskeletal Stability at the Drosophila Neuromuscular Junction.
From: Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
The Journal of neuroscience : the official journal of the Society for Neuroscience
- Publish Date: Sep 2006
- ISSN: 1529-2401
- Volume: 26
- Issue: 36
- Pages: 9293-303
- Medium: Internet
- Language: English
- Citation (JAMA): Viquez Natasha M, Li Caroline R, Wairkar Yogesh P, et al. The B' Protein Phosphatase 2a Regulatory Subunit Well-rounded Regulates Synaptic Growth and Cytoskeletal Stability at the Drosophila Neuromuscular Junction.. J. Neurosci. Sep 2006;26:9293-303
Abstract
Synaptic growth is essential for the development and plasticity of neural circuits. To identify molecular mechanisms regulating synaptic growth, we performed a gain-of-function screen for synapse morphology mutants at the Drosophila neuromuscular junction (NMJ). We isolated a B’ regulatory subunit of protein phosphatase 2A (PP2A) that we have named well-rounded (wrd). Neuronal overexpression of wrd leads to overgrowth of the synaptic terminal. Endogenous Wrd protein is present in the larval nervous system and muscle and is enriched at central and neuromuscular synapses. wrd is required for normal synaptic development; in its absence, there are fewer synaptic boutons and there is a decrease in synaptic strength. wrd functions presynaptically to promote normal synaptic growth and postsynaptically to maintain normal levels of evoked transmitter release. In the absence of wrd, the presynaptic cytoskeleton is abnormal, with an increased proportion of unbundled microtubules. Reducing PP2A enzymatic activity also leads to an increase in unbundled microtubules, an effect enhanced by reducing wrd levels. Hence, wrd promotes the function of PP2A and is required for normal cytoskeletal organization, synaptic growth, and synaptic function at the Drosophila NMJ.
Mesh Headings (Keywords): Animals, Cell Enlargement, Cells, Cultured, Cytoskeleton, Drosophila, Enzyme Activation, Neuromuscular Junction, Phosphoprotein Phosphatases, Protein Phosphatase 2, Synapses
Check for Full Text / PubMed Unique Identifier (PMID): 16957085
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