Quantitative and Microscopic Methods for Studying the Endocytic Pathway.
From: Center for Biochemistry, University of Cologne, Germany.
Methods in molecular biology (Clifton, N.J.)
- Publish Date: 2006
- ISSN: 1064-3745
- Volume: 346
- Issue:
- Pages: 423-38
- Medium: Print
- Language: English
- Citation (JAMA): Rivero Francisco, Maniak Markus, et al. Quantitative and Microscopic Methods for Studying the Endocytic Pathway.. Methods Mol. Biol. 2006;346:423-38
Abstract
Endocytosis is a process that is essential to the life of all eukaryotic cells. Laboratory strains of Dictyostelium are extremely efficient in the uptake of both particles and fluid. Many different cellular processes feed into the endocytic pathway, and many organelle-associated and cytoplasmic proteins, including the ones from the cytoskeleton, contribute to the efficiency of transit. Therefore mutants, especially in genes of unknown function, must be characterized regarding their endocytic performance. We describe the most common tools and protocols to visualize and quantify all of the individual steps in endocytic transit.
Mesh Headings (Keywords): Acid Phosphatase, Animals, Dictyostelium, Endocytosis, Escherichia coli, Exocytosis, Lysosomes, Microscopy, Fluorescence, Phagocytosis, Pinocytosis, Saccharomyces cerevisiae, alpha-Mannosidase
Check for Full Text / PubMed Unique Identifier (PMID): 16957305
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.