Assaying Cell-cell Adhesion.
From: Department of Clinical and Biological Sciences, University of Turin, Torino, Italy.
Methods in molecular biology (Clifton, N.J.)
- Publish Date: 2006
- ISSN: 1064-3745
- Volume: 346
- Issue:
- Pages: 449-67
- Medium: Print
- Language: English
- Citation (JAMA): Bozzaro Salvatore, et al. Assaying Cell-cell Adhesion.. Methods Mol. Biol. 2006;346:449-67
Abstract
A major feature in Dictyostelium development is the transition from the unicellular to the multicellular stage, a process brought about by chemotaxis and cell-cell adhesion. Growth-phase cells are weakly cohesive, whereas aggregation-competent cells adhere strongly to each other. In addition, aggregating cells display an ethylenediamine tetraacetic acid (EDTA)-resistant form of adhesion, which is developmentally regulated. Measuring cell-cell adhesion can thus be a simple and convenient method by which to assess the developmental progression of cells, to characterize mutants, and to discriminate between development and functional defects in cell adhesion molecules or membrane-cytoskeletal interactions. A quantitative cell adhesion assay is obviously crucial for identifying novel cell adhesion factors.
Mesh Headings (Keywords): Animals, Biological Assay, Cell Adhesion, Cell Adhesion Molecules, Cell Aggregation, Dictyostelium, Light, Scattering, Radiation
Check for Full Text / PubMed Unique Identifier (PMID): 16957307
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.