Novel Upper Meta-pathway Extradiol Dioxygenase Gene Diversity in Polluted Soil.
From: Department of Biological and Environmental Sciences, General Microbiology, 00014 University of Helsinki, Finland.
FEMS microbiology ecology
- Publish Date: Oct 2006
- ISSN: 0168-6496
- Volume: 58
- Issue: 1
- Pages: 134-44
- Medium: Print
- Language: English
- Citation (JAMA): Sipilä Timo P, Riisiö Heikki, Yrjälä Kim, et al. Novel Upper Meta-pathway Extradiol Dioxygenase Gene Diversity in Polluted Soil.. FEMS Microbiol. Ecol. Oct 2006;58:134-44
Abstract
For the determination of the catabolic community diversity that is related to biodegradation potential, we developed a protocol for the assessment of catabolic marker genes in polluted soils. Primers specific to upper pathway extradiol dioxygenase genes were designed which amplified a 469-bp product from Sphingomonas sp. HV3. The constructed primers were used in PCR amplification of upper pathway ring cleavage genes from DNA directly isolated from a mineral oil polluted landfill site, a mineral oil landfarming site and a birch rhizosphere-associated soil that was either artificially polluted with a PAH mixture or not polluted. Amplicons were cloned and subjected to restriction fragment length polymorphism analysis dividing the HhaI-digested products into operational taxonomic units. Altogether 26 different operational taxonomic units were detected with the sequence similarity to known catabolic genes of Alpha-, Beta-, and Gammaproteobacteria. Phylogenetic analysis divided the operational taxonomic units from the polluted soils into seven clusters. Two contained exclusively sequences with no close homologues in the database, therefore representing novel catabolic genes. This large proportion of novel extradiol sequences shows that there is an extensive unknown catabolic diversity in polluted environments.
Mesh Headings (Keywords): Bacteria, DNA, Bacterial, Environmental Pollution, Molecular Sequence Data, Oxygenases, Phylogeny, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, Soil Microbiology, Sphingomonas
Check for Full Text / PubMed Unique Identifier (PMID): 16958914
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