Codon Usage Bias and Trna Over-expression in Buchnera Aphidicola After Aromatic Amino Acid Nutritional Stress on Its Host Acyrthosiphon Pisum.
From: Laboratoire de Biologie Fonctionnelle Insectes et Interactions, UMR INRA/INSA de Lyon, 203 Bâtiment Louis Pasteur, 69621 Villeurbanne Cedex, France. hubert.charles@insa-lyon.fr
Nucleic acids research
- Publish Date: 2006
- ISSN: 1362-4962
- Volume: 34
- Issue: 16
- Pages: 4583-92
- Medium: Internet
- Language: English
- Citation (JAMA): Charles Hubert, Calevro Federica, Vinuelas José, et al. Codon Usage Bias and Trna Over-expression in Buchnera Aphidicola After Aromatic Amino Acid Nutritional Stress on Its Host Acyrthosiphon Pisum.. Nucleic Acids Res. 2006;34:4583-92
Abstract
Codon usage bias and relative abundances of tRNA isoacceptors were analysed in the obligate intracellular symbiotic bacterium, Buchnera aphidicola from the aphid Acyrthosiphon pisum, using a dedicated 35mer oligonucleotide microarray. Buchnera is archetypal of organisms living with minimal metabolic requirements and presents a reduced genome with high-evolutionary rate. Codonusage in Buchnera has been overcome by the high mutational bias towards AT bases. However, several lines of evidence for codon usage selection are given here. A significant correlation was found between tRNA relative abundances and codon composition of Buchnera genes. A significant codon usage bias was found for the choice of rare codons in Buchnera: C-ending codons are preferred in highly expressed genes, whereas G-ending codons are avoided. This bias is not explained by GC skew in the bacteria and might correspond to a selection for perfect matching between codon-anticodon pairs for some essential amino acids in Buchnera proteins. Nutritional stress applied to the aphid host induced a significant overexpression of most of the tRNA isoacceptors in bacteria. Although, molecular regulation of the tRNA operons in Buchnera was not investigated, a correlation between relative expression levels and organization in transcription unit was found in the genome of Buchnera.
Mesh Headings (Keywords): Animals, Anticodon, Aphids, Buchnera, Codon, Cytosine, Diet, Gene Expression Regulation, Bacterial, Guanine, Oligonucleotide Array Sequence Analysis, Phenylalanine, RNA, Transfer, Tyrosine
Check for Full Text / PubMed Unique Identifier (PMID): 16963497
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