Template Requirements for Recognition and Copying by Sindbis Virus Rna-dependent Rna Polymerase.
From: Department of Biology, Indiana University, 1001 E. Third Street, Bloomington, IN 47405, USA.
Virology
- Publish Date: Feb 2007
- ISSN: 0042-6822
- Volume: 358
- Issue: 1
- Pages: 221-32
- Medium: Print
- Language: English
- Citation (JAMA): Thal Melissa A, Wasik Brian R, Posto Jessica, et al. Template Requirements for Recognition and Copying by Sindbis Virus Rna-dependent Rna Polymerase.. Virology Feb 2007;358:221-32
Abstract
The Sindbis virus (SIN) nonstructural protein nsP4 possesses the RNA-dependent RNA polymerase activity required for the replication of the SIN genome and transcription of a subgenomic mRNA during infection. Isolation of this protein from other viral components of the RNA synthetic complex allowed the characterization of template requirements for nsP4-mediated genome replication. The major findings of this study are: (i) in the absence of other viral proteins nsP4 is capable of copying SIN plus- and minus-strand templates, but does not transcribe subgenomic RNA; (ii) mutations in the 3’ conserved sequence element and poly(A) tail of the plus-strand template prevent nsP4-mediated de novo initiation of minus-strand RNA synthesis; (iii) nsP4-dependent terminal addition of nucleotides occurs on template RNA possessing certain mutations in the 3’CSE and polyadenylate tail ; (iv) nsP4 is capable of minus-strand synthesis independent of the sequence at the 5’ end of the template; (v) an A-U rich sequence in the 3’CSE represents a binding site for a replicase component, probably nsP4; (vi) plus-strand genomic RNA synthesis is dependent on the 3’ end of the minus-strand template. These studies begin to define the specific interactions with the viral RNA templates mediated by individual components of the viral replication complex and suggest a model for ternary complex formation during the initiation of minus-strand RNA synthesis.
Mesh Headings (Keywords): 3’ Untranslated Regions, 5’ Untranslated Regions, Animals, Binding Sites, Cell Line, Cricetinae, Mutation, Nucleotides, RNA, Viral, Sindbis Virus, Templates, Genetic, Transcription, Genetic, Viral Nonstructural Proteins
Check for Full Text / PubMed Unique Identifier (PMID): 16979682
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