Optimization of Procedures for Cloning by Somatic Cell Nuclear Transfer in Mice.
From: Advanced Cell Technology, Worcester, MA, USA.
Methods in molecular biology (Clifton, N.J.)
- Publish Date: 2006
- ISSN: 1064-3745
- Volume: 348
- Issue:
- Pages: 111-24
- Medium: Print
- Language: English
- Citation (JAMA): Chung Young Gie, Gao Shaorong, Latham Keith E, et al. Optimization of Procedures for Cloning by Somatic Cell Nuclear Transfer in Mice.. Methods Mol. Biol. 2006;348:111-24
Abstract
Cloning by somatic cell nuclear transfer is a complex procedure that is dependent on correct interactions between oocyte and donor cell genome. These interactions require minimal insult to either the oocyte or the transplanted nucleus. Available data also indicate that reprogramming the donor cell genome may be slow, so that the cloned embryo expresses genes typical of the donor cell, and thus has different characteristics from normal embryos. Procedures that minimize damage to the donor genome and that address the unique characteristics of the cloned construct should enhance the efficacy of the method.
Mesh Headings (Keywords): Animals, Cells, Cultured, Cloning, Organism, Embryo Culture Techniques, Epigenesis, Genetic, Fertilization in Vitro, Genomic Imprinting, Mice, Nuclear Transfer Techniques
Check for Full Text / PubMed Unique Identifier (PMID): 16988375
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