In Vitro Culture of C. Elegans Somatic Cells.
From: Department of Anesthesiology, Vanderbilt University Medical Center, Nashville, TN, USA.
Methods in molecular biology (Clifton, N.J.)
- Publish Date: 2006
- ISSN: 1064-3745
- Volume: 351
- Issue:
- Pages: 265-73
- Medium: Print
- Language: English
- Citation (JAMA): Strange Kevin, Morrison Rebecca, et al. In Vitro Culture of C. Elegans Somatic Cells.. Methods Mol. Biol. 2006;351:265-73
Abstract
Because of technical hurdles, large-scale cell culture methods have not been widely exploited until recently for the study of Caenorhabditis elegans. Culturing differentiated cells from larvae and adult worms is probably not technically feasible because of difficulties in removing the animal’s cuticle and dissociating cells. In contrast, large numbers of developing embryo cells can be isolated relatively easily. When placed in culture, embryo cells undergo terminal differentiation within 24 h. Cultured embryo cells have been used recently to characterize ion channel function and regulation and to determine cell specific gene expression patterns. This chapter will provide a detailed description of the methods for isolating and culturing C. elegans embryo cells.
Mesh Headings (Keywords): Animals, Caenorhabditis elegans, Cell Culture Techniques, Cell Fractionation, Cells, Cultured, Embryo, Nonmammalian, Ion Channels
Check for Full Text / PubMed Unique Identifier (PMID): 16988440
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.