Isolation and Characterization of an Infectious Replication-competent Molecular Clone of Ecotropic Porcine Endogenous Retrovirus Class C.
From: Paul-Ehrlich-Institut, Paul-Ehrlich-Strasse 51-59, D-63225 Langen, Germany.
Journal of virology
- Publish Date: Oct 2006
- ISSN: 0022-538X
- Volume: 80
- Issue: 20
- Pages: 10258-61
- Medium: Print
- Language: English
- Citation (JAMA): Preuss Thomas, Fischer Nicole, Boller Klaus, et al. Isolation and Characterization of an Infectious Replication-competent Molecular Clone of Ecotropic Porcine Endogenous Retrovirus Class C.. J. Virol. Oct 2006;80:10258-61
Abstract
Xenotransplantation of pig organs is complicated by the existence of polytropic replication-competent porcine endogenous retroviruses (PERV) capable of infecting human cells. The potential for recombination between ecotropic PERV-C and human-tropic PERV-A and PERV-B adds another level of infectious risk. Proviral PERV-C were characterized in MAX-T cells derived from d/d haplotype miniature swine. Three proviruses were cloned from a genomic library. Clone PERV-C(1312) generated infectious particles after transfection into porcine ST-IOWA cells. Electron microscopy revealed the same morphologies of virions in MAX-T cells and in ST-IOWA cells infected with cell-free PERV-C(1312) particles, indicating that MAX-T cells harbor one functional PERV-C provirus.
Mesh Headings (Keywords): Animals, Cell Line, Cloning, Molecular, Endogenous Retroviruses, Microscopy, Electron, Transmission, Proviruses, Swine, Transfection, Virion, Virus Replication
Check for Full Text / PubMed Unique Identifier (PMID): 17005704
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