Immunization Against Anthrax Using Bacillus Subtilis Spores Expressing the Anthrax Protective Antigen.
From: Royal Holloway University of London, Egham, Surrey TW20 0EX, UK.
Vaccine
- Publish Date: Jan 2007
- ISSN: 0264-410X
- Volume: 25
- Issue: 2
- Pages: 346-55
- Medium: Print
- Language: English
- Citation (JAMA): Duc Le H, Hong Huynh A, Atkins Helen S, et al. Immunization Against Anthrax Using Bacillus Subtilis Spores Expressing the Anthrax Protective Antigen.. Vaccine Jan 2007;25:346-55
Abstract
Protective immunity to anthrax can be achieved by antibodies raised against the secreted protective antigen (PA) and this forms the basis of the current acellular vaccines for human use. Bacillus subtilis spores have previously been used for delivery of heterologous antigens by the oral and nasal routes and their intrinsic heat-stability make them attractive vaccine vehicles. In this study we have expressed PA, or segments of PA, in B. subtilis using two strategies. First, display on the spore coat, and second, in the germinated spore (or vegetative cell). Using parenteral delivery we show that recombinant spores can be used to confer protective immunity in a murine model using an in vitro toxin neutralization assay and a challenge experiment with the latter showing protection to 100 median lethal dose of B. anthracis spores. PA must be secreted from the live bacterium or alternatively displayed on the spore surface to confer protective immunity. Intracellular expression of PA failed to confer protective immunity. The highest levels of protective immunity were achieved when PA was displayed on the spore surface as well as in the germinating spore.
Mesh Headings (Keywords): Animals, Anthrax, Anthrax Vaccines, Antibodies, Bacterial, Antigens, Bacterial, Bacterial Toxins, Female, Immunization, Immunoglobulin G, Mice, Mice, Inbred BALB C, Neutralization Tests, Spores, Bacterial, Vaccines, Synthetic
Check for Full Text / PubMed Unique Identifier (PMID): 17007969
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.