Adenoviral Infection Induces a Multi-faceted Innate Cellular Immune Response That is Mediated by the Toll-like Receptor Pathway in A549 Cells.
From: Division of Medical Genetics, Department of Pediatrics, Duke University Medical Center, Durham, North Carolina 27710, USA.
Virology
- Publish Date: Feb 2007
- ISSN: 0042-6822
- Volume: 358
- Issue: 2
- Pages: 357-72
- Medium: Print
- Language: English
- Citation (JAMA): Hartman Zachary C, Black Esther P, Amalfitano Andrea, et al. Adenoviral Infection Induces a Multi-faceted Innate Cellular Immune Response That is Mediated by the Toll-like Receptor Pathway in A549 Cells.. Virology Feb 2007;358:357-72
Abstract
Adenovirus vectors are known to induce certain genes and impact innate response networks, but a broad understanding of this process and its mechanisms is currently lacking. For this reason, we chose to investigate and characterize Ad innate immunity using homogeneous, primary MEF cells replete with all the elements of the pathogen-sensing Toll-Like Receptor (TLR) pathway. By using an array-based approach to maximally define transcriptome changes induced upon Ad vector infection, we discovered that Ad infection induces a potent gene and transcription factor network response. This response is characterized by significant changes in the expression of genes involved in focal adhesion, tight junction, and RNA regulation, in addition to TLR pathway and other innate sensing genes. Further investigation using human A549 cells knocked down for various TLR pathway adaptors, revealed significant impacts on the Ad initiation of NF-kB and interferon responses, thus confirming TLR involvement in Ad-mediated immunity across diverse species.
Mesh Headings (Keywords): Adenoviridae, Adenoviridae Infections, Animals, Cell Line, Tumor, Cells, Cultured, Cytokines, Fibroblasts, Gene Expression Profiling, Genetic Vectors, Humans, Immunity, Natural, Interferons, Mice, Mice, Inbred C57BL, NF-kappa B, Oligonucleotide Array Sequence Analysis, Toll-Like Receptors, Transduction, Genetic
Check for Full Text / PubMed Unique Identifier (PMID): 17027060
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