Identification of Two Isoforms of Dsk2-related Protein Xdrp1 in Xenopus Eggs.
From: Department of Molecular Biology, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan.
Biochemical and biophysical research communications
- Publish Date: Nov 2006
- ISSN: 0006-291X
- Volume: 350
- Issue: 3
- Pages: 768-73
- Medium: Print
- Language: English
- Citation (JAMA): Tanaka Kanae, Funakoshi Minoru, Inoue Kazue, et al. Identification of Two Isoforms of Dsk2-related Protein Xdrp1 in Xenopus Eggs.. Biochem. Biophys. Res. Commun. Nov 2006;350:768-73
Abstract
The budding yeast UbL-UBA protein Dsk2 has a UbL domain at its N-terminus and a UBA domain at its C-terminus, and thus functions as a shuttle protein in the ubiquitin-proteasome pathway. In this report we describe two isoforms of Xenopus Dsk2-related protein, XDRP1L and XDRP1S. Difference of the two proteins in sequence was that the UbL domain of XDRP1S lacks 15 residues in the middle part of that of XDRP1L. Both XDRP1L and XDRP1S were expressed in Xenopus eggs. XDRP1L and XDRP1S bound to polyubiquitinated proteins via their UBA domains. XDRP1L also bound to the proteasome via its UbL domain, whereas the XDRP1S UbL domain was less likely to bind to the proteasome. Instead, XDRP1S not XDRP1L bound to monomeric cyclin A and prevented its degradation. The existence of such Dsk2-isoforms in Xenopus eggs suggests that the shuttling function via the UbL-UBA protein Dsk2 is evolutionally conserved across species.
Mesh Headings (Keywords): Amino Acid Sequence, Animals, Cell Cycle Proteins, Molecular Sequence Data, Nuclear Proteins, Oocytes, Phosphoproteins, Protein Isoforms, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, Ubiquitins, Xenopus Proteins, Xenopus laevis
Check for Full Text / PubMed Unique Identifier (PMID): 17027914
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