Germination-independent Induction of Cellular Immune Response by Bacillus Subtilis Spores Displaying the C Fragment of the Tetanus Toxin.
From: Department of Structural and Functional Biology, Federico II University of Napoli, via Cinthia, Complesso MSA, 80126 Napoli, Italy.
Vaccine
- Publish Date: Jan 2007
- ISSN: 0264-410X
- Volume: 25
- Issue: 5
- Pages: 788-93
- Medium: Print
- Language: English
- Citation (JAMA): Mauriello Emilia M F, Cangiano Giuseppina, Maurano Francesco, et al. Germination-independent Induction of Cellular Immune Response by Bacillus Subtilis Spores Displaying the C Fragment of the Tetanus Toxin.. Vaccine Jan 2007;25:788-93
Abstract
Bacillus subtilis spores displaying the tetanus toxin fragment C (TTFC) on their surface have been previously shown to induce the production of specific IgG and secretory IgA in mice immunized through the oral or nasal route. Aim of this study was to analyze whether these spores were also able to induce cellular immunity, and whether such immune response was dependent on spore germination in the animal gastro-intestinal tract (GIT). We first developed a germination defective strain of B. subtilis unable to produce viable cells inside the mouse GIT. Germination-defective and congenic wild-type spores both expressing TTFC on their surface were then used to orally immunize Balb/C mice. Both types of spores induced spleen and mesenteric lymph nodes cell proliferation as well as production of IFNgamma but not of IL-4 and IL-10 in both districts. Our results indicate that recombinant spores preferentially induce a strong cell-mediated immune response with a Th1 phenotype, independently from their ability to germinate in the GIT.
Mesh Headings (Keywords): Animals, Bacillus subtilis, Bacterial Vaccines, Female, Germination, Interferon Type II, Interleukin-10, Interleukin-4, Lymphocyte Activation, Mice, Mice, Inbred BALB C, Peptide Fragments, Spores, Bacterial, Tetanus Toxin
Check for Full Text / PubMed Unique Identifier (PMID): 17028079
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.