Identification of a Cytotoxic T-lymphocyte (Ctl) Epitope Recognized by Gag-specific Ctls in Cynomolgus Monkeys Infected with Simian/Human Immunodeficiency Virus.
From: Department of Infectious, Parasitic and Immune-mediated Diseases, Istituto Superiore di Sanità , Viale Regina Elena 299, 00161 Rome, Italy.
The Journal of general virology
- Publish Date: Nov 2006
- ISSN: 0022-1317
- Volume: 87
- Issue: Pt 11
- Pages: 3385-92
- Medium: Print
- Language: English
- Citation (JAMA): Negri Donatella R M, Borghi Martina, Baroncelli Silvia, et al. Identification of a Cytotoxic T-lymphocyte (Ctl) Epitope Recognized by Gag-specific Ctls in Cynomolgus Monkeys Infected with Simian/Human Immunodeficiency Virus.. J. Gen. Virol. Nov 2006;87:3385-92
Abstract
Infection of Macaca fascicularis (cynomolgus monkey) with chimeric simian/human immunodeficiency virus (SHIV) provides a valuable experimental animal model of AIDS and is widely used for the development of human immunodeficiency virus vaccine strategies. In these settings, analysis of CD8(+) T-cell responses during infection represents one of the key parameters for monitoring the evaluation of containment of virus replication. The generation of Gag-specific CD8(+) T cells was reported previously from a cynomolgus monkey infected with SHIV89.6P by taking advantage of a B-lymphoblastoid cell line transduced with a retroviral vector expressing simian immunodeficiency virus (SIV) Gag. Here, it was shown that these cytotoxic T lymphocytes (CTLs) demonstrated specificity for a single 9 aa peptide (NCVGDHQAA) spanning aa 192-200 of the SIVmac239 p55(gag) protein. Furthermore, a positive response was found against the same epitope in one of six other SHIV-infected monkeys. This newly identified SIV Gag CTL epitope in SHIV-infected cynomolgus monkeys will be a useful tool for monitoring and evaluating Gag-specific immune responses during vaccination and infection in the cynomolgus monkey model of AIDS.
Mesh Headings (Keywords): Animals, CD8-Positive T-Lymphocytes, Cells, Cultured, Cytotoxicity, Immunologic, Epitopes, T-Lymphocyte, Gene Products, gag, HIV, Interferon Type II, Leukocytes, Mononuclear, Macaca fascicularis, Peptides, Reassortant Viruses, Simian Acquired Immunodeficiency Syndrome, Simian immunodeficiency virus, Species Specificity, T-Lymphocyte Subsets, T-Lymphocytes, Cytotoxic
Check for Full Text / PubMed Unique Identifier (PMID): 17030874
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