Intracellular Zinc Elevation Measured with a "Calcium-specific" Indicator During Ischemia and Reperfusion in Rat Hippocampus: a Question on Calcium Overload.
From: Department of Biomedical Science, Program in Molecular and Cellular Biology, Ohio University, Athens, Ohio 45701, USA.
The Journal of neuroscience : the official journal of the Society for Neuroscience
- Publish Date: Oct 2006
- ISSN: 1529-2401
- Volume: 26
- Issue: 41
- Pages: 10430-7
- Medium: Internet
- Language: English
- Citation (JAMA): Stork Christian J, Li Yang V, et al. Intracellular Zinc Elevation Measured with a "Calcium-specific" Indicator During Ischemia and Reperfusion in Rat Hippocampus: a Question on Calcium Overload.. J. Neurosci. Oct 2006;26:10430-7
Abstract
Much of our current evidence concerning of the role of calcium (Ca2+) as a second messenger comes from its interaction with fluorescent probes; however, many Ca2+ probes also have a higher affinity for another divalent cation: zinc (Zn2+). In this study, using a selective Zn2+ probe (Newport Green), we investigated the accumulation of intracellular Zn2+ transients in acute rat hippocampal slices during ischemia, simulated by oxygen and glucose deprivation (OGD). Subsequent reperfusion with glucose-containing oxygenated medium resulted in an additional increase in intracellular Zn2+. Such observations compelled us to investigate the contribution of Zn2+ to the alleged intracellular Ca2+ overload occurring in ischemia and reperfusion. Using confocal fluorescent microscopy of Calcium Green-1, a widely used Ca2+ indicator, we detected increases in fluorescence intensity during OGD and reperfusion. However, application of a Zn2+ chelator, at the peak of the fluorescence elevation (interpreted as Ca2+ overload), resulted in a significant drop in intensity, suggesting that rising Zn2+ is the primary source of the increasing Calcium Green-1 fluorescence. Finally, staining with the cell viability indicator propidium iodide revealed that Zn2+ is responsible for the ischemic neuronal cell death, because Zn2+ chelation prevented cells from sustaining ischemic damage. Current cellular models of ischemic injury center on Ca2+-mediated excitotoxicity. Our results indicate that Zn2+ elevation contributes to conventionally recognized Ca2+ overload and also suggest that the role of Ca2+ in neurotoxicity described previously using Ca2+ probes may need to be re-examined to determine whether effect previously attributed to Ca2+ could, in part, be attributable to Zn2+.
Mesh Headings (Keywords): Animals, Calcium, Cell Death, Cell Hypoxia, Hippocampus, Intracellular Fluid, Male, Rats, Rats, Sprague-Dawley, Reperfusion, Zinc
Check for Full Text / PubMed Unique Identifier (PMID): 17035527
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