Isolation and Identification of Proangiotensin-12, a Possible Component of the Renin-angiotensin System.
From: Circulatory and Body Fluid Regulation, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan.
Biochemical and biophysical research communications
- Publish Date: Dec 2006
- ISSN: 0006-291X
- Volume: 350
- Issue: 4
- Pages: 1026-31
- Medium: Print
- Language: English
- Citation (JAMA): Nagata Sayaka, Kato Johji, Sasaki Kazuki, et al. Isolation and Identification of Proangiotensin-12, a Possible Component of the Renin-angiotensin System.. Biochem. Biophys. Res. Commun. Dec 2006;350:1026-31
Abstract
The renin-angiotensin (RA) system plays an important role in regulating blood pressure and fluid balance. In the search for bioactive peptides with an antibody binding to the N-terminal portion of angiotensin II (Ang II), we isolated a new angiotensinogen-derived peptide from the rat small intestine. Consisting of 12 amino acids, this peptide was termed proangiotensin-12 based on its possible role of an Ang II precursor. Proangiotensin-12 constricted aortic strips and, when infused intravenously, raised blood pressure in rats, while both the vasoconstrictor and pressor response to proangiotensin-12 were abolished by captopril and by CV-11974, an Ang II type I receptor blocker. Proangiotensin-12 is abundant in a wide range of organs and tissues including the small intestine, spleen, kidneys, and liver of rats. The identification of proangiotensin-12 suggests a processing cascade of the RA system, different from the cleavage of angiotensinogen to Ang I by renin.
Mesh Headings (Keywords): Amino Acid Sequence, Angiotensinogen, Animals, Molecular Sequence Data, Organ Specificity, Peptide Fragments, Rabbits, Rats, Renin-Angiotensin System, Tissue Distribution
Check for Full Text / PubMed Unique Identifier (PMID): 17045572
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