Neogenesis and Proliferation of Beta-cells Induced by Human Betacellulin Gene Transduction Via Retrograde Pancreatic Duct Injection of an Adenovirus Vector.
From: Department of Internal Medicine and Molecular Science, Graduate School of Medicine, Osaka University, 2-2-B5, Yamadaoka, Suita-city, Osaka 565-0871, Japan. ytokui@imed2.med.osaka-u.ac.jp
Biochemical and biophysical research communications
- Publish Date: Dec 2006
- ISSN: 0006-291X
- Volume: 350
- Issue: 4
- Pages: 987-93
- Medium: Print
- Language: English
- Citation (JAMA): Tokui Yae, Kozawa Junji, Yamagata Kazuya, et al. Neogenesis and Proliferation of Beta-cells Induced by Human Betacellulin Gene Transduction Via Retrograde Pancreatic Duct Injection of an Adenovirus Vector.. Biochem. Biophys. Res. Commun. Dec 2006;350:987-93
Abstract
Betacellulin (BTC) has been shown to have a role in the differentiation and proliferation of beta-cells both in vitro and in vivo. We administered a human betacellulin (hBTC) adenovirus vector to male ICR mice via retrograde pancreatic duct injection. As a control, we administered a beta-galactosidase adenovirus vector. In the mice, hBTC protein was mainly overexpressed by pancreatic duct cells. On immunohistochemical analysis, we observed features of beta-cell neogenesis as newly formed insulin-positive cells in the duct cell lining or islet-like cell clusters (ICCs) closely associated with the ducts. The BrdU labeling index of beta-cells was also increased by the betacellulin vector compared with that of control mice. These results indicate that hBTC gene transduction into adult pancreatic duct cells promoted beta-cell differentiation (mainly from duct cells) and proliferation of pre-existing beta-cells, resulting in an increase of the beta-cell mass that improved glucose tolerance in diabetic mice.
Mesh Headings (Keywords): Adenoviridae, Animals, Cell Differentiation, Cell Proliferation, Cells, Cultured, Insulin-Secreting Cells, Intercellular Signaling Peptides and Proteins, Intracellular Signaling Peptides and Proteins, Male, Mice, Mice, Inbred ICR, Microinjections, Pancreatic Ducts, Transduction, Genetic
Check for Full Text / PubMed Unique Identifier (PMID): 17046717
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