Bacterial Survival in Evaporating Deposited Droplets on a Teflon-coated Surface.
From: Department of Mechanical Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong Special Administrative Region, People’s Republic of China.
Applied microbiology and biotechnology
- Publish Date: Dec 2006
- ISSN: 0175-7598
- Volume: 73
- Issue: 3
- Pages: 703-12
- Medium: Print
- Language: English
- Citation (JAMA): Xie Xiaojian, Li Yuguo, Zhang Tong, et al. Bacterial Survival in Evaporating Deposited Droplets on a Teflon-coated Surface.. Appl. Microbiol. Biotechnol. Dec 2006;73:703-12
Abstract
Understanding of bacterial survival in aerosols is crucial for controlling infection transmission via airborne aerosols and/or large droplets routes. The cell viability changes of four bacteria species (Escherichia coli K12 JM109; Acinetobacter sp. 5A5; Pseudomonas oleovorans X5; and Staphylococcus aureus X8), three Gram-negative and one Gram-positive, in a large evaporating droplet of size 1,800 microm in diameter on teflon-coated slides were measured using the LIVE/DEAD BacLight solution and a microscope. Droplets of three levels of salinity (0, 0.9, and 36% w/v) were tested. All four species survived well during the droplet evaporation process, but died mostly at the time when droplets were dried out at 40-45 min. The final bacteria survival rate after droplets were completely dried was dependent on bacteria species and the salinity of the suspension solution. Droplet evaporation over the first 35-40 min had no adverse effect on bacterial survival for the droplets tested. The lethal effect of desiccation was found to be the most important death mechanism.
Mesh Headings (Keywords): Acinetobacter, Aerosols, Bacteria, Bacterial Physiology, Colony Count, Microbial, Desiccation, Escherichia coli, Kinetics, Microbial Viability, Models, Biological, Polytetrafluoroethylene, Sodium Chloride, Staphylococcus aureus, Surface Properties, Water, Water Microbiology
Check for Full Text / PubMed Unique Identifier (PMID): 17053902
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