Co-expression of Human Abcg5 and Abcg8 in Insect Cells Generates an Androstan Stimulated Membrane Atpase Activity.
From: National Medical Center, Institute of Haematology and Immunology, Membrane Research Group of the Hungarian Academy of Sciences, H-1113 Budapest, Hungary.
FEBS letters
- Publish Date: Nov 2006
- ISSN: 0014-5793
- Volume: 580
- Issue: 26
- Pages: 6139-44
- Medium: Print
- Language: English
- Citation (JAMA): Müller Marianna, Klein Izabella, Kopácsi Szilárd, et al. Co-expression of Human Abcg5 and Abcg8 in Insect Cells Generates an Androstan Stimulated Membrane Atpase Activity.. FEBS Lett. Nov 2006;580:6139-44
Abstract
Mutations in the ATP-binding cassette (ABC) proteins ABCG5 or ABCG8 cause sitosterolemia, a condition with increased accumulation of plant sterols. Upon high level expression of the ABCG5 and ABCG8 proteins in baculovirus-Sf9 cell expression system we found a distinct, vanadate sensitive ATPase activity in isolated membrane preparations only when the two proteins were co-expressed. This ATPase activity was significantly stimulated by the addition of certain androgen hormones and analogs, and was effectively inhibited by progesterone. Our results provide a new aspect of biochemical and functional characterization of the ABCG5/ABCG8 proteins and their possible involvement in steroid hormone transport or regulation.
Mesh Headings (Keywords): ATP-Binding Cassette Transporters, Adenosine Triphosphatases, Androgens, Androstanes, Animals, Cell Line, Hormones, Humans, Insects, Lipoproteins, Membrane Proteins, Progesterone, Transduction, Genetic, Vanadates
Check for Full Text / PubMed Unique Identifier (PMID): 17055487
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