Mucosal Administration of Ag85b-esat-6 Protects Against Infection with Mycobacterium Tuberculosis and Boosts Prior Bacillus Calmette-guerin Immunity.
From: Department of Infectious Disease Immunology, Statens Serum Institute, Artillerivej 5, DK-2300 Copenhagen, Denmark. JDI@ssi.dk
Journal of immunology (Baltimore, Md. : 1950)
- Publish Date: Nov 2006
- ISSN: 0022-1767
- Volume: 177
- Issue: 9
- Pages: 6353-60
- Medium: Print
- Language: English
- Citation (JAMA): Dietrich Jes, Andersen Claire, Rappuoli Rino, et al. Mucosal Administration of Ag85b-esat-6 Protects Against Infection with Mycobacterium Tuberculosis and Boosts Prior Bacillus Calmette-guerin Immunity.. J. Immunol. Nov 2006;177:6353-60
Abstract
We have examined the intranasal administration of a vaccine against Mycobacterium tuberculosis (M.tb) consisting of the mucosal adjuvant LTK63 and the Ag Ag85B-ESAT-6. Vaccination with LTK63/Ag85B-ESAT-6 gave a strong and sustained Th1 response mediated by IFN-gamma-secreting CD4 cells, which led to long-lasting protection against tuberculosis, equivalent to that observed with bacillus Calmette-Guérin (BCG) or Ag85B-ESAT-6 in dimethyldioctadecylammonium bromide/monophosphoryl lipid A. Because a crucial element of novel vaccine strategies is the ability to boost BCG-derived immunity, we also tested whether LTK63/Ag85B-ESAT-6 could act as a BCG booster vaccine in BCG-vaccinated mice. We found that vaccinating with LTK63/Ag85B-ESAT-6 strongly boosted prior BCG-stimulated immunity. Compared with BCG-vaccinated nonboosted mice, we observed that infection with M.tb led to a significant increase in anti-M.tb-specific CD4 T cells in the lungs of LTK63/Ag85B-ESAT-6-boosted animals. This correlated with a significant increase in the protection against M.tb in LTK63/Ag85B-ESAT-6-boosted mice, compared with BCG-vaccinated animals. Thus, LTK63/Ag85B-ESAT-6 represents an efficient preventive vaccine against tuberculosis with a strong ability to boost prior BCG immunity.
Mesh Headings (Keywords): Acyltransferases, Administration, Intranasal, Animals, Antigens, Bacterial, Bacterial Proteins, Bacterial Toxins, Enterotoxins, Escherichia coli Proteins, Immunization, Secondary, Mice, Mice, Inbred Strains, Mycobacterium bovis, Mycobacterium tuberculosis, Nasal Mucosa, Recombinant Fusion Proteins, T-Lymphocytes, Tuberculosis Vaccines, Tuberculosis, Pulmonary, Vaccination
Check for Full Text / PubMed Unique Identifier (PMID): 17056566
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