Ketogenic Diet Protects the Hippocampus from Kainic Acid Toxicity by Inhibiting the Dissociation of Bad from 14-3-3.
From: Department of Anatomy and Neurobiology, Institute of Health Science, Medical Research Center for Neural Dysfunction, Gyeongsang National University, Jinju, Gyeongnam, South Korea.
Journal of neuroscience research
- Publish Date: Dec 2006
- ISSN: 0360-4012
- Volume: 84
- Issue: 8
- Pages: 1829-36
- Medium: Print
- Language: English
- Citation (JAMA): Noh Hae Sook, Kim Yoon Sook, Kim Young Hee, et al. Ketogenic Diet Protects the Hippocampus from Kainic Acid Toxicity by Inhibiting the Dissociation of Bad from 14-3-3.. J. Neurosci. Res. Dec 2006;84:1829-36
Abstract
The ketogenic diet (KD) is often effective for intractable epilepsy, but its antiepileptic mechanisms remain largely unknown. Within the cell death/survival pathway, Akt and its downstream protein Bad play an important role in kainic acid (KA)-induced cell death. Therefore, we investigated the effects of a KD on KA-induced changes in the Akt/Bad/14-3-3 signaling pathway by evaluating Akt, Bad, 14-3-3, and cleaved caspase-3 expression levels as well as their relative interactions. Our results showed that a KD did not affect the expression levels of Akt, Bad, Bcl-xL, Bax, and 14-3-3 but increased phospho-Akt [serine 473; p-Akt (Ser473)] and phospho-Bad [serine 136; p-Bad (Ser136)] expression levels as well as decreased cleaved caspase-3 levels following a KA-induced seizure in the hippocampus. Furthermore, we found that a KD increased the protein-protein interaction between 14-3-3 and p-Bad (Ser136), which might be phosphorylated by p-Akt (Ser473), and decreased interaction of Bad and Bcl-xL. These results suggest that a KD might protect, at least partially, the hippocampus from KA-induced cell death via inhibiting the dissociation of Bad from 14-3-3.
Mesh Headings (Keywords): 14-3-3 Proteins, Animals, Blotting, Western, Brain Injuries, Caspase 3, Diet, Carbohydrate-Restricted, Gene Expression Regulation, Hippocampus, Immunoprecipitation, Kainic Acid, Male, Mice, Mice, Inbred ICR, bcl-Associated Death Protein
Check for Full Text / PubMed Unique Identifier (PMID): 17058267
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