Expression of T-cadherin in Tumor Cells Influences Invasive Potential of Human Hepatocellular Carcinoma.
From: Inserm U602, Université Paris XI, Villejuif, France.
The FASEB journal : official publication of the Federation of American Societies for Experimental Biology
- Publish Date: Nov 2006
- ISSN: 1530-6860
- Volume: 20
- Issue: 13
- Pages: 2291-301
- Medium: Internet
- Language: English
- Citation (JAMA): Riou Philippe, Saffroy Raphael, Chenailler Catherine, et al. Expression of T-cadherin in Tumor Cells Influences Invasive Potential of Human Hepatocellular Carcinoma.. FASEB J. Nov 2006;20:2291-301
Abstract
Overexpression of T-cadherin (T-cad) transcripts occurs in approximately 50% of human hepatocellular carcinomas (HCCs). To elucidate T-cad functions in HCC, we examined T-cad protein expression in normal and tumoral human livers and hepatoma cell lines and investigated its influence on invasive potential of HCC using RNA interference silencing of T-cad expression in Mahlavu cells. Whereas T-cad expression was restricted to endothelial cells (EC) from large blood vessels in normal livers, it was up-regulated in sinusoidal EC from 8/15 invasive HCCs. Importantly, in three of them (38%) T-cad was detected in tumor cells within regions in which E-cadherin expression was absent. Among six hepatoma cell lines, only Mahlavu expressed T-cad but not E-cadherin. T-cad exhibited a globally punctuate distribution in quiescent Mahlavu and additionally it concentrated at the leading edge of migrating cells. Matrigel invasion assay revealed that Mahlavu possess a high invasive potential that was significantly inhibited by T-cad silencing. Wound healing and random motility assays demonstrated that inhibition of T-cad expression in Mahlavu significantly reduced their motility. We propose that T-cad expression in tumor cells might occur by cadherin-switching during epithelial-mesenchymal transition and may represent an additional mechanism contributing to HCC metastasis.
Mesh Headings (Keywords): Animals, Cadherins, Carcinoma, Hepatocellular, Cell Culture Techniques, Cell Division, Cell Line, Tumor, Cell Movement, DNA Primers, Endothelial Cells, Fibroblasts, Hepatocytes, Humans, Liver, Liver Neoplasms, Neoplasm Invasiveness, RNA, Small Interfering, Rabbits, Transcription, Genetic, Transfection, Wound Healing
Check for Full Text / PubMed Unique Identifier (PMID): 17077306
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