Molecular Characterization of a New Porcine Rotavirus P Genotype Found in an Asymptomatic Pig in Slovenia.
From: Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Zaloska 4, SI-1104 Ljubljana, Slovenia. andrej.steyer@mf.uni-lj.si
Virology
- Publish Date: Mar 2007
- ISSN: 0042-6822
- Volume: 359
- Issue: 2
- Pages: 275-82
- Medium: Print
- Language: English
- Citation (JAMA): Steyer Andrej, Poljsak-Prijatelj Mateja, Barlic-Maganja Darja, et al. Molecular Characterization of a New Porcine Rotavirus P Genotype Found in an Asymptomatic Pig in Slovenia.. Virology Mar 2007;359:275-82
Abstract
Rotaviral RNA was detected in the stool sample of an asymptomatic fattening pig at a Slovenian pig farm. To characterize the rotavirus, RT-PCR was used, employing primers specific for the VP7, VP4 and NSP4 genes. Specific products were purified and the sequencing reaction was performed for the molecular analysis of amplified genes. Nucleotide and amino acid sequences of the VP7 gene were found highly identical (85.3-88.1% and 90.7-91.6%) to G1 genotype strains. Phylogenetic and molecular analyses of the VP7 antigen regions revealed the sample to be from a new lineage of G1 genotype. In the molecular analysis of the VP4 gene, only 70.9% nucleotide (76.2% amino acid) identity was found with the most related rotavirus VP4 gene from GenBank. Following this, the NSP4 gene was also analyzed. After the phylogenetic analysis, it clustered with the NSP4 B genotype, but also seemed to represent a new lineage of this genotype. This new rotavirus strain, named P21-5, differed greatly from all rotaviruses characterized so far in all three genes analyzed. The virulence of this strain is not clear yet and has to be investigated.
Mesh Headings (Keywords): Amino Acid Sequence, Animals, Feces, Genotype, Molecular Sequence Data, Phylogeny, Rotavirus, Slovenia, Swine, Viral Proteins
Check for Full Text / PubMed Unique Identifier (PMID): 17078990
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