Characterization of Pericentrin Isoforms in Vivo.
From: Department of Brain Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama 700-8558, Japan. miyoshi@cc.okayama-u.ac.jp
Biochemical and biophysical research communications
- Publish Date: Dec 2006
- ISSN: 0006-291X
- Volume: 351
- Issue: 3
- Pages: 745-9
- Medium: Print
- Language: English
- Citation (JAMA): Miyoshi Ko, Asanuma Masato, Miyazaki Ikuko, et al. Characterization of Pericentrin Isoforms in Vivo.. Biochem. Biophys. Res. Commun. Dec 2006;351:745-9
Abstract
Pericentrin was first identified as a mouse centrosomal protein and is now referred to as pericentrin A. A larger homologous protein in humans with a C-terminal calmodulin-binding domain was later identified as pericentrin B. Pericentrin has been shown to be one of the key components in ciliogenesis, but in vivo pericentrin products have remained ambiguous. Here we characterized pericentrin isoforms in mice. Two pericentrin transcripts of 9.5 and 6.9 kb were recognized on the mouse tissue Northern blots, while a cRNA probe for a 5’-terminal sequence shared by pericentrin A and B failed to hybridize to the 6.9-kb message. Two pericentrin cDNAs were identified, which encoded pericentrin B and a novel isoform, pericentrin S, sharing with pericentrin B a C-terminal calmodulin-binding motif. Three pericentrin proteins of 360, 255, and 250 kDa revealed by immunoprecipitation analysis were thought to correspond to pericentrin B, pericentrin S, and an unknown N-terminal product.
Mesh Headings (Keywords): Amino Acid Sequence, Animals, Antigens, Base Sequence, Binding Sites, Mice, Molecular Sequence Data, Organ Specificity, Protein Binding, Tissue Distribution
Check for Full Text / PubMed Unique Identifier (PMID): 17084386
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.