Two Modes of Microtubule Sliding Driven by Cytoplasmic Dynein.
From: Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba 3-8-1, Meguro-ku, Tokyo 153-8902, Japan.
Proceedings of the National Academy of Sciences of the United States of America
- Publish Date: Nov 2006
- ISSN: 0027-8424
- Volume: 103
- Issue: 47
- Pages: 17736-40
- Medium: Print
- Language: English
- Citation (JAMA): Shima Tomohiro, Kon Takahide, Imamula Kenji, et al. Two Modes of Microtubule Sliding Driven by Cytoplasmic Dynein.. Proc. Natl. Acad. Sci. U.S.A. Nov 2006;103:17736-40
Abstract
Dynein is a huge multisubunit microtubule (MT)-based motor, whose motor domain resides in the heavy chain. The heavy chain comprises a ring of six AAA (ATPases associated with diverse cellular activities) modules with two slender protruding domains, the tail and stalk. It has been proposed that during the ATP hydrolysis cycle, this tail domain swings against the AAA ring as a lever arm to generate the power stroke. However, there is currently no direct evidence to support the model that the tail swing is tightly linked to dynein motility. To address the question of whether the power stroke of the tail drives MT sliding, we devised an in vitro motility assay using genetically biotinylated cytoplasmic dyneins anchored on a glass surface in the desired orientation with a biotin-streptavidin linkage. Assays on the dyneins with the site-directed biotin tag at eight different locations provided evidence that robust MT sliding is driven by the power stroke of the tail. Furthermore, the assays revealed slow MT sliding independent of dynein orientation on the glass surface, which is mechanically distinct from the sliding driven by the power stroke of the tail.
Mesh Headings (Keywords): Animals, Biological Assay, Biotin, Cytoplasm, Dictyostelium, Dynein ATPase, Microtubules, Models, Molecular, Protein Structure, Tertiary, Recombinant Fusion Proteins, Streptavidin
Check for Full Text / PubMed Unique Identifier (PMID): 17085593
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