Mitogen Stimulation Activates Different Signaling Pathways in Early- and Late-divided T Cells As Revealed by Cdna Microarray Analysis.
From: Graduate School of Biomedical Science, UT Health Science Center at Houston, Houston, TX 77030, USA.
International journal of molecular medicine
- Publish Date: Dec 2006
- ISSN: 1107-3756
- Volume: 18
- Issue: 6
- Pages: 1127-39
- Medium: Print
- Language: English
- Citation (JAMA): Li Yufeng, Wong Kwong-Kwok, Matsueda Satoko, et al. Mitogen Stimulation Activates Different Signaling Pathways in Early- and Late-divided T Cells As Revealed by Cdna Microarray Analysis.. Int. J. Mol. Med. Dec 2006;18:1127-39
Abstract
Mobilization of tumor-reactive CD8+ T cells remains the major challenge of cancer immunotherapy. Knowing how and when the T cell response expands and differentiates after antigen stimulation would make a significant contribution to the development of tumor vaccines. In the current study, we used CFSE-based cell sorting and cDNA microarray to identify the gene expression profile of adjacent generations of T cells after PHA stimulation. Early-divided generations of T cells responded to stimulation by activating cell cycle and surviving gene pathways, while late generations of T cells had more dramatic changes in transcription of cytokine genes. Reconstruction of biochemical pathways, activated in both early and late generations of T cells, also confirmed the impact of division in focal-adhesion kinases. Because most tumors are infiltrated by lymphocytes, our studies indicate a novel approach to identify ‘systemic biological responses’ of T cells, which could determine the design, and optimization of effective tumor vaccines.
Mesh Headings (Keywords): Cell Division, Cell Size, Cells, Cultured, DNA, Complementary, Flow Cytometry, Fluorescein, Fluorescent Dyes, Gene Expression Profiling, Humans, Kinetics, Leukocytes, Mononuclear, Mitogens, Oligonucleotide Array Sequence Analysis, Phytohemagglutinins, Signal Transduction, T-Lymphocytes
Check for Full Text / PubMed Unique Identifier (PMID): 17089018
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