Alpha4-integrin(+) Endothelium Derived from Primate Embryonic Stem Cells Generates Primitive and Definitive Hematopoietic Cells.
From: Department of Pediatrics, Graduate School of Medicine, Kyoto University, Japan.
Blood
- Publish Date: Mar 2007
- ISSN: 0006-4971
- Volume: 109
- Issue: 6
- Pages: 2406-15
- Medium: Print
- Language: English
- Citation (JAMA): Shinoda Gen, Umeda Katsutsugu, Heike Toshio, et al. Alpha4-integrin(+) Endothelium Derived from Primate Embryonic Stem Cells Generates Primitive and Definitive Hematopoietic Cells.. Blood Mar 2007;109:2406-15
Abstract
The mechanism of commencement of hematopoiesis in blood islands of the yolk sac and the aorta-gonad-mesonephros (AGM) region during primate embryogenesis remains elusive. In this study, we demonstrated that VE-cadherin(+)CD45(-) endothelial cells derived from nonhuman primate embryonic stem cells are able to generate primitive and definitive hematopoietic cells sequentially, as revealed by immunostaining of floating erythrocytes and colony-forming assay in cultures. Single bipotential progenitors for hematopoietic and endothelial lineages are included in this endothelial cell population. Furthermore, hemogenic activity of these endothelial cells is observed exclusively in the alpha4-integrin(+) subpopulation; bipotential progenitors are 4-fold enriched in this subpopulation. The kinetics of this hemogenic subpopulation is similar to that of hemogenic endothelial cells previously reported in the yolk sac and the AGM region in vivo in that they emerge for only a limited time. We suggest that VE-cadherin(+)CD45(-)alpha4-integrin(+) endothelial cells are involved in primitive and definitive hematopoiesis during primate embryogenesis, though VE-cadherin(-)CD45(-)alpha4-integrin(+) cells are the primary sources for primitive hematopoiesis.
Mesh Headings (Keywords): Animals, Antigens, CD, Antigens, CD45, Cadherins, Cells, Cultured, Embryonic Stem Cells, Endothelial Cells, Endothelium, Erythrocytes, Hematopoiesis, Integrin alpha4, Macaca fascicularis
Check for Full Text / PubMed Unique Identifier (PMID): 17090646
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