Chromatin Loading of Smc5/6 is Induced by Dna Replication but Not by Dna Double-strand Breaks.
From: Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Miyagi 980-8578, Japan.
Biochemical and biophysical research communications
- Publish Date: Dec 2006
- ISSN: 0006-291X
- Volume: 351
- Issue: 4
- Pages: 935-9
- Medium: Print
- Language: English
- Citation (JAMA): Tsuyama Takashi, Inou Katsutoshi, Seki Masayuki, et al. Chromatin Loading of Smc5/6 is Induced by Dna Replication but Not by Dna Double-strand Breaks.. Biochem. Biophys. Res. Commun. Dec 2006;351:935-9
Abstract
Smc6, a member of the structural maintenance of chromosomes (SMC) family of proteins, forms a complex with related Smc5. Genetic analyses of yeast have demonstrated the involvement of Smc6 in DNA repair and checkpoint responses. In this study, we investigated the role of the Smc5/6 complex in higher eukaryotes by analyzing its behavior in Xenopus laevis egg extracts. Smc5/6 was loaded onto chromatin during DNA replication in a manner dependent on the initiation of DNA synthesis, and it dissociated from chromatin during mitosis. Moreover, the induction of DNA double-strand breaks following replication did not significantly affect the amount of chromatin-associated Smc6. These findings suggest that the Smc5/6 complex is regulated during the cell cycle, presumably in anticipation of DNA damage that may arise during replication.
Mesh Headings (Keywords): Animals, Chromatin, Chromosomal Proteins, Non-Histone, DNA Breaks, Double-Stranded, DNA Damage, DNA Replication, G2 Phase, Mitosis, S Phase, Xenopus Proteins, Xenopus laevis
Check for Full Text / PubMed Unique Identifier (PMID): 17094943
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