N-cadherin is a Useful Marker for the Progenitor of Cardiomyocytes Differentiated from Mouse Es Cells in Serum-free Condition.
From: Department of Biological Sciences, Graduate School of Sciences, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Biochemical and biophysical research communications
- Publish Date: Dec 2006
- ISSN: 0006-291X
- Volume: 351
- Issue: 4
- Pages: 877-82
- Medium: Print
- Language: English
- Citation (JAMA): Honda Masahiko, Kurisaki Akira, Ohnuma Kiyoshi, et al. N-cadherin is a Useful Marker for the Progenitor of Cardiomyocytes Differentiated from Mouse Es Cells in Serum-free Condition.. Biochem. Biophys. Res. Commun. Dec 2006;351:877-82
Abstract
Cardiomyocytes are known to differentiate spontaneously from embryonic stem (ES) cells when they formed aggregates, so called “embryoid bodies”, in the presence of serum. In this study, we explored the induction of cardiomyocytes from mouse ES cells in chemically defined serum-free medium by using a mesoderm-inducing factor, BMP4. Comparing the different inductive methods, we found a candidate cell surface marker, N-cadherin, for cardiomyocyte progenitors from ES cells. N-cadherin-positive cells highly expressed cardiogenic markers, Nkx2.5, Tbx5, and Isl1, and showed a high differentiation rate into cardiomyocyte lineage. These results indicate that N-cadherin can be a useful cell surface marker for the progenitors of cardiomyocyte differentiated from ES cells in the serum-free culture.
Mesh Headings (Keywords): Animals, Biological Markers, Bone Morphogenetic Proteins, Cadherins, Cell Differentiation, Cell Membrane, Cells, Cultured, Embryonic Stem Cells, Hepatocyte Nuclear Factor 3-beta, Mice, Myocytes, Cardiac, Polymerase Chain Reaction
Check for Full Text / PubMed Unique Identifier (PMID): 17097609
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