Characterization of Cd133+ Hepatocellular Carcinoma Cells As Cancer Stem/Progenitor Cells.
From: Department of Gastroenterology, Gifu University Graduate School of Medicine, Gifu 501 1194, Japan.
Biochemical and biophysical research communications
- Publish Date: Dec 2006
- ISSN: 0006-291X
- Volume: 351
- Issue: 4
- Pages: 820-4
- Medium: Print
- Language: English
- Citation (JAMA): Suetsugu Atsushi, Nagaki Masahito, Aoki Hitomi, et al. Characterization of Cd133+ Hepatocellular Carcinoma Cells As Cancer Stem/Progenitor Cells.. Biochem. Biophys. Res. Commun. Dec 2006;351:820-4
Abstract
The CD133 antigen, identified as a hematopoietic stem cell marker, appears in various human embryonic epithelia including the neural tube, gut, and kidney. We herein investigated whether CD133(+) cells isolated from human hepatocellular carcinoma cell lines possess cancer stem/progenitor cell-like properties. Among the three cell lines studied, the CD133 antigen was found to be expressed only on the surface of Huh-7 cells. CD133(+) cells from Huh-7 performed a higher in vitro proliferative potential and lower mRNA expressions of mature hepatocyte markers, glutamine synthetase and cytochrome P450 3A4, than CD133(-) population of Huh-7 cells. When either CD133(+) or CD133(-) cells were subcutaneously injected into SCID mice, CD133(+) cells formed tumors, whereas CD133(-) cells induced either a very small number of tumors or none at all. Taken together, the identification of CD133(+) cells could thus be a potentially powerful tool to investigate the tumorigenic process in the hepatoma system and to also develop effective therapies targeted against hepatocellular carcinoma.
Mesh Headings (Keywords): Animals, Antigens, CD, Carcinoma, Hepatocellular, Glycoproteins, Humans, Liver Neoplasms, Mice, Mice, SCID, Neoplasm Transplantation, Peptides, Stem Cells
Check for Full Text / PubMed Unique Identifier (PMID): 17097610
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.