Induction of Necrotic Cell Death and Mitochondrial Permeabilization by Heme Binding Protein 2/Soul.
From: Institute of Oncotherapy, University of Pécs, Pécs, Hungary.
FEBS letters
- Publish Date: Nov 2006
- ISSN: 0014-5793
- Volume: 580
- Issue: 27
- Pages: 6447-54
- Medium: Print
- Language: English
- Citation (JAMA): Szigeti Andras, Bellyei Szabolcs, Gasz Balazs, et al. Induction of Necrotic Cell Death and Mitochondrial Permeabilization by Heme Binding Protein 2/Soul.. FEBS Lett. Nov 2006;580:6447-54
Abstract
We found that heme-binding protein 2/SOUL sensitised NIH3T3 cells to cell death induced by A23187 and etoposide, but it did not affect reactive oxygen species formation. In the presence of sub-threshold calcium, recombinant SOUL provoked mitochondrial permeability transition (mPT) in vitro that was inhibited by cyclosporine A (CsA). This effect was verified in vivo by monitoring the dissipation of mitochondrial membrane potential. Flow cytometry analysis showed that SOUL promoted necrotic death in A23187 and etoposide treated cells, which effect was prevented by CsA. These data suggest that besides its heme-binding properties SOUL promotes necrotic cell death by inducing mPT.
Mesh Headings (Keywords): Animals, Antineoplastic Agents, Phytogenic, Calcimycin, Calcium, Carrier Proteins, Cyclosporine, Etoposide, Hemeproteins, Humans, Immunosuppressive Agents, Ionophores, Mice, Mitochondria, Mitochondrial Membranes, NIH 3T3 Cells, Necrosis, Permeability, Pregnancy Proteins, Recombinant Proteins
Check for Full Text / PubMed Unique Identifier (PMID): 17098234
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