Hyaline Cartilage Formation and Enchondral Ossification Modeled with Kum5 and Op9 Chondroblasts.
From: Department of Reproductive Biology and Pathology, National Institute for Child and Health Development, Tokyo 157-8535, Japan.
Journal of cellular biochemistry
- Publish Date: Apr 2007
- ISSN: 0730-2312
- Volume: 100
- Issue: 5
- Pages: 1240-54
- Medium: Print
- Language: English
- Citation (JAMA): Sugiki Tadashi, Uyama Taro, Toyoda Masashi, et al. Hyaline Cartilage Formation and Enchondral Ossification Modeled with Kum5 and Op9 Chondroblasts.. J. Cell. Biochem. Apr 2007;100:1240-54
Abstract
What is it that defines a bone marrow-derived chondrocyte? We attempted to identify marrow-derived cells with chondrogenic nature and immortality without transformation, defining “immortality” simply as indefinite cell division. KUM5 mesenchymal cells, a marrow stromal cell line, generated hyaline cartilage in vivo and exhibited enchondral ossification at a later stage after implantation. Selection of KUM5 chondroblasts based on the activity of the chondrocyte-specific cis-regulatory element of the collagen alpha2(XI) gene resulted in enhancement of their chondrogenic nature. Gene chip analysis revealed that OP9 cells, another marrow stromal cell line, derived from macrophage colony-stimulating factor-deficient osteopetrotic mice and also known to be niche-constituting cells for hematopoietic stem cells expressed chondrocyte-specific or -associated genes such as type II collagen alpha1, Sox9, and cartilage oligomeric matrix protein at an extremely high level, as did KUM5 cells. After cultured OP9 micromasses exposed to TGF-beta3 and BMP2 were implanted in mice, they produced abundant metachromatic matrix with the toluidine blue stain and formed type II collagen-positive hyaline cartilage within 2 weeks in vivo. Hierarchical clustering and principal component analysis based on microarray data of the expression of cell surface markers and cell-type-specific genes resulted in grouping of KUM5 and OP9 cells into the same subcategory of “chondroblast,” that is, a distinct cell type group. We here show that these two cell lines exhibit the unique characteristics of hyaline cartilage formation and enchondral ossification in vitro and in vivo.
Mesh Headings (Keywords): Animals, Biological Markers, Bone Development, Bone Morphogenetic Proteins, Cells, Cultured, Chondrocytes, Chondrogenesis, Collagen Type II, Gene Expression Profiling, Growth Plate, Hyaline Cartilage, Mesenchymal Stem Cells, Mice, Mice, Inbred BALB C, Mice, Nude, Oligonucleotide Array Sequence Analysis, Osteogenesis, Stromal Cells, Transforming Growth Factor beta, Transforming Growth Factor beta3
Check for Full Text / PubMed Unique Identifier (PMID): 17115412
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