Protein Tyrosine Phosphorylation of a Heparin-binding Sperm Membrane Mitogen (Hbsm) is Associated with Capacitation and Acrosome Reaction.
From: Gamete Immunology Laboratory, Indian Institute of Chemical Biology, 4 Raja S.C Mullick Road, Jadavpur, Kolkata 700032, W.B., India.
Biochemical and biophysical research communications
- Publish Date: Jan 2007
- ISSN: 0006-291X
- Volume: 352
- Issue: 2
- Pages: 404-9
- Medium: Print
- Language: English
- Citation (JAMA): Mor Visesato, Das Tapasi, Bhattacharjee Maitreyi, et al. Protein Tyrosine Phosphorylation of a Heparin-binding Sperm Membrane Mitogen (Hbsm) is Associated with Capacitation and Acrosome Reaction.. Biochem. Biophys. Res. Commun. Jan 2007;352:404-9
Abstract
Protein tyrosine phosphorylation in spermatozoa is associated with epididymal maturation and though to be central for attainment of a capacitated state and expression of hyperactivated motility. Heparin, the most highly sulfated glycosaminoglycans, was also the most potent at stimulating the acrosomal reaction in bovine epididymal spermatozoa. Studies using radiolabeled inorganic phosphate showed 11-fold increase (32)Pi incorporation in heparin-binding sperm membrane protein (HBSM) during spermatozoal capacitation, and the phosphorylation occurs at the tyrosine residue. Epididymal spermatozoa were induced to undergo capacitation and acrosome reaction by 70% when the cells were incubated in BWW medium supplemented with heparin. The spermatozoa pre-treated with anti-HBSM antibody showed 46% reduction in the hyperactivated motility and lowers the acrosome reaction. This was confirms by measuring the hydrolysis of benzoyl-l-arginine ethyl ether (BAEE) by the acrosomal enzyme; acrosin. The preliminary finding suggests that HBSM may play an important role in the sperm capacitation and acrosome reaction.
Mesh Headings (Keywords): Acrosome, Acrosome Reaction, Animals, Cell Membrane, Cells, Cultured, Dose-Response Relationship, Drug, Goats, Heparin, Male, Membrane Proteins, Mitogen-Activated Protein Kinases, Phosphorylation, Protein-Tyrosine Kinases, Sperm Capacitation, Spermatozoa
Check for Full Text / PubMed Unique Identifier (PMID): 17126299
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.