Buthionine Sulfoximine Causes Endothelium Dependent Hyper-relaxation and Hypoadiponectinemia.
From: Department of Endocrinology and Metabolism, Dokkyo University School of Medicine, Mibu, Tochigi, Japan.
Life sciences
- Publish Date: Feb 2007
- ISSN: 0024-3205
- Volume: 80
- Issue: 9
- Pages: 873-8
- Medium: Print
- Language: English
- Citation (JAMA): Iwata Chigusa, Wang Xi, Uchida Kohsuke, et al. Buthionine Sulfoximine Causes Endothelium Dependent Hyper-relaxation and Hypoadiponectinemia.. Life Sci. Feb 2007;80:873-8
Abstract
A close relationship between oxidative stress, endothelial dysfunction, and hypoadiponectinemia has been observed. The present study was performed to investigate how glutathione depletion via buthionine sulfoximine (BSO) administration affects endothelial function and adiponectin levels in rats. Acetylcholine (Ach)-induced vasodilation was significantly enhanced in BSO-treated rats, compared with control rats. This was completely abolished by L-NAME, and Ach-induced vasodilation was not observed in the aorta without endothelium. These results suggest that Ach-induced hyper-relaxation of the aorta in BSO-treated rats is completely dependent on the presence of endothelium and mediated by changes in eNOS activity. Catalase significantly inhibited this relaxation to Ach and no effect of catalase on sodium nitroprusside-induced relaxation of the aorta without endothelium was observed in BSO-treated rats. Thus, hyper-relaxation of the aorta in BSO-treated rats is likely caused by H2O2 in addition to NO produced by the endothelium via an eNOS-dependent mechanism. Hypoadiponectinemia and decreased levels of adiponectin mRNA in adipose tissue were observed in BSO-treated rats. Protein expression of eNOS and SODs (SOD-1 and SOD-2) in the aorta was increased and plasma NOx levels were decreased in BSO-treated rats. Our results suggest that oxidative stress induced by BSO causes eNOS uncoupling and hyper-relaxation by producing H2O2, and that BSO-induced oxidative stress causes hypoadiponectinemia, probably by increasing H2O2 production in adipose tissue.
Mesh Headings (Keywords): Adiponectin, Adipose Tissue, Animals, Aorta, Thoracic, Biopterin, Blood Pressure, Buthionine Sulfoximine, Enzyme Inhibitors, Glutathione, Glutathione Synthase, Heart Rate, Hydrogen Peroxide, Immunoblotting, Liver, Male, Nitric Oxide, Oxidative Stress, Rats, Rats, Sprague-Dawley, Reverse Transcriptase Polymerase Chain Reaction, Vasodilation
Check for Full Text / PubMed Unique Identifier (PMID): 17137603
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