Pattern of Distribution of Calcitonin Gene-related Peptide in the Dorsal Root Ganglion of Animal Models of Diabetes Mellitus.
From: Department of Anatomy, Faculty of Medicine and Health Sciences, UAE University, PO Box 17666, Al Ain, United Arab Emirates. eadeghate@uaeu.ac.ae
Annals of the New York Academy of Sciences
- Publish Date: Nov 2006
- ISSN: 0077-8923
- Volume: 1084
- Issue:
- Pages: 296-303
- Medium: Print
- Language: English
- Citation (JAMA): Adeghate Ernest, Rashed Hameed, Rajbandari Satyan, et al. Pattern of Distribution of Calcitonin Gene-related Peptide in the Dorsal Root Ganglion of Animal Models of Diabetes Mellitus.. Ann. N. Y. Acad. Sci. Nov 2006;1084:296-303
Abstract
This article examined the pattern of distribution of calcitonin gene-related peptide (CGRP) in the dorsal root ganglion (DRG) of normal and diabetic Wistar, Zucker lean, and Goto-Kakizaki (GK) rats to determine whether there are changes in the number and pattern of distribution of CGRP-positive neurons after the onset of latent or overt diabetes. Type 1 diabetes mellitus was induced in Wistar rats by a single dose of streptozotocin (STZ) given intraperitoneally (60 mg/kg body weight). Four weeks after the induction of diabetes mellitus, diabetic (n = 6) and normal (n = 6), Zucker lean (n = 6), and GK (n = 6) rats were anesthetized with chloral hydrate and their DRGs were removed and processed for immunohistochemistry. CGRP-positive neurons were observed in the DRG of normal and diabetic Wistar, Zucker lean (nondiabetic), and GK (animal model of type 2 diabetes) rats. CGRP was present in small-, medium-, and large-sized neurons of the DRG in these three animal models. Only a small percentage of large-sized neurons contains CGRP. The number of CGRP-positive neurons was significantly (P < 0.05) reduced in STZ-induced diabetic Wistar and GK rats compared to normal Wistar and Zucker lean rats. Moreover, the quantity of CGRP-containing varicose nerves was less in diabetic Wistar and GK rats compared to control Wistar and Zucker lean rats. The reduced number of CGRP-positive neurons in the DRG of GK rats indicated that subjects with latent diabetes may already have dysfunctional CGRP metabolism and thus diabetic neuropathy.
Mesh Headings (Keywords): Animals, Calcitonin Gene-Related Peptide, Diabetes Mellitus, Experimental, Diabetic Neuropathies, Disease Models, Animal, Ganglia, Spinal, Immunohistochemistry, Male, Rats, Rats, Mutant Strains, Rats, Wistar, Rats, Zucker, Reference Values
Check for Full Text / PubMed Unique Identifier (PMID): 17151309
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