Influence of Monensin on Cation Influx and Na+ -k+ -atpase Activity of Eimeria Tenella Sporozoites in Vitro.
From: Department of Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100094, PR China.
The Journal of parasitology
- Publish Date: Oct 2006
- ISSN: 0022-3395
- Volume: 92
- Issue: 5
- Pages: 1092-6
- Medium: Print
- Language: English
- Citation (JAMA): Wang Zhuo, Suo Xun, Xia Xi, et al. Influence of Monensin on Cation Influx and Na+ -k+ -atpase Activity of Eimeria Tenella Sporozoites in Vitro.. J. Parasitol. Oct 2006;92:1092-6
Abstract
The experiments were conducted to determine intrasporozoite Na+/K+ concentrations (by AAS) and membrane-bound Na+ -K+ -ATPase activity (measured by UV-VIS with a Na+ -K+ -ATPase Detection Kit) of Eimeria tenella sporozoites of the sensitive line (i.e., the parent line, coded as OS) and 2 resistant lines, derived from the parent line (coded as OR125 and OR200), with and without in vitro exposure to monensin. These parameters for OR125 and OR200 were significantly lower than those for OS. In vitro exposure to monensin increased intrasporozoite Na+/K+ concentrations and Na+ -K+ -ATPase activity, but the stimulation on OS was significantly higher than those on OR125 and OR200, indicating that monensin had less effect on resistant parasites. The results of this study suggest that altered biochemical or physiological properties, or both, in the membranes of E. tenella might be related to a reduced sensitivity to monensin.
Mesh Headings (Keywords): Animals, Chickens, Coccidiosis, Coccidiostats, Dose-Response Relationship, Drug, Drug Resistance, Eimeria tenella, Monensin, Potassium, Random Allocation, Sodium, Sodium-Potassium-Exchanging ATPase, Sporozoites
Check for Full Text / PubMed Unique Identifier (PMID): 17152956
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.