Ultrastructure of Babesia Wa1 (Apicomplexa: Piroplasma) During Infection of Erythrocytes in a Hamster Model.
From: Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine/Kansas State University, 1800 Denison Avenue, Manhattan, Kansas 66506-5606, USA.
The Journal of parasitology
- Publish Date: Oct 2006
- ISSN: 0022-3395
- Volume: 92
- Issue: 5
- Pages: 1104-7
- Medium: Print
- Language: English
- Citation (JAMA): Braga W, Venasco J, Willard L, et al. Ultrastructure of Babesia Wa1 (Apicomplexa: Piroplasma) During Infection of Erythrocytes in a Hamster Model.. J. Parasitol. Oct 2006;92:1104-7
Abstract
Babesia Washington-1 (WA1) is a newly identified intraerythrocyte infectious agent of human babesiosis in the western United States. The purpose of the present study is to describe the ultrastructural changes in affected erythrocytes during the infectious process in a susceptible animal model, the golden Syrian hamster. Two, 1-mo-old female hamsters were inoculated intraperitoneally (i.p.) with 1.8 x 10(9) Babesia WA1-infected erythrocytes originally isolated from a human case and serially passaged in hamsters. Saphenous vein blood samples (20 microl) were collected at 0, 24, 36, 48, 60, 72, 84, and 96 hr postinoculation (PI). Parasitemia was determined at each time interval by quick staining of blood smears showing 0, 2.5, 5, 10, 12.5, 22.5, 70, and almost 100% parasitemic erythrocytes at the corresponding PI time interval, respectively. Animals showed weakness and dehydration 72 hr PI inoculation, and were killed by 96 hr PI. Selected blood samples from 0, 24, 48, 72, and 96 hr were fixed in cacodylate buffer, dehydrated in ethanol gradients, resin embedded, and then thin sectioned and stained with uranyl acetate and lead citrate for transmission electron microscopy or gold-coated for scanning electron microscopy (SEM). Shape and surface membrane changes in erythrocytes were demonstrated by SEM and were more evident at 72 and 96 hr PI. Infected erythrocytes underwent changes in shape 24 hr PI, from few protrusions to several perforations, some of them resembling a “swiss cheese” appearance 96 hr PI. Several erythrocytes had irregular surface membranes and Babesia WA1 organisms were seen at different stages of development within erythrocytes, from single trophozoites to several merozoites (young trophozoites), some of them dividing to form typical tetrads. In general, Babesia WAI induced severe morphological changes in the erythrocytes, and these changes were more evident in almost all infected cells 96 hr PI.
Mesh Headings (Keywords): Animals, Babesia, Babesiosis, Cricetinae, Disease Models, Animal, Erythrocyte Membrane, Erythrocytes, Female, Humans, Mesocricetus, Microscopy, Electron, Scanning, Microscopy, Electron, Transmission, Vacuoles, Virulence
Check for Full Text / PubMed Unique Identifier (PMID): 17152960
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.