Pbk/Topk Promotes Tumour Cell Proliferation Through P38 Mapk Activity and Regulation of the Dna Damage Response.
From: Cell Biology Laboratory, Department of Biochemistry, Biosciences Institute, National University of Ireland, Cork, Ireland.
Oncogene
- Publish Date: May 2007
- ISSN: 0950-9232
- Volume: 26
- Issue: 24
- Pages: 3451-61
- Medium: Print
- Language: English
- Citation (JAMA): Ayllón V, O'connor R, et al. Pbk/Topk Promotes Tumour Cell Proliferation Through P38 Mapk Activity and Regulation of the Dna Damage Response.. Oncogene May 2007;26:3451-61
Abstract
The contribution of the insulin-like growth-factor-I receptor (IGF-IR) to tumour progression is well documented. To identify new mediators of IGF-IR function in cancer, we recently isolated genes differentially expressed in cells overexpressing the IGF-IR. Among these was the serine/threonine kinase PBK/TOPK (PDZ-binding kinase/T-LAK cell-originated protein kinase), previously associated with highly proliferative cells and tissues. Here, we show that PBK is expressed at high levels in tumour cell lines compared with non-transformed cells. IGF-I could induce PBK expression only in transformed cells, whereas epidermal growth factor could induce PBK in non-transformed MCF-10A breast epithelial cells. Suppression of PBK expression using small interfering RNA did not prevent progression through the cell cycle, but caused decreased proliferation over time in culture, and reduced clonogenic growth in soft agarose. PBK knockdown impaired p38 activation after long-term stimulation with different growth factors and reduced DU145 cells motility. Suppressed PBK expression also resulted in an impaired response to DNA damage that was evident by the decreased generation of gamma-H2AX, increased DNA damage and decreased cell survival. Taken together, the data indicate that PBK is necessary for appropriate activation and function of the p38 pathway by growth factors. Thus, enhanced expression of PBK may facilitate tumour growth by mediating p38 activation and by helping cells to overcome DNA damage.
Mesh Headings (Keywords): Animals, Carcinogenicity Tests, Cell Cycle, Cell Line, Tumor, Cell Movement, Cell Proliferation, DNA Damage, Enzyme Inhibitors, Epidermal Growth Factor, Histones, Humans, Insulin-Like Growth Factor I, Protein-Serine-Threonine Kinases, Receptor, IGF Type 1, Signal Transduction, Ultraviolet Rays, p38 Mitogen-Activated Protein Kinases
Check for Full Text / PubMed Unique Identifier (PMID): 17160018
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