Association of Nuclear Pore Fg-repeat Domains to Ntf2 Import and Export Complexes.
From: Department of Physics, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Journal of molecular biology
- Publish Date: Feb 2007
- ISSN: 0022-2836
- Volume: 366
- Issue: 1
- Pages: 330-45
- Medium: Print
- Language: English
- Citation (JAMA): Isgro Timothy A, Schulten Klaus, et al. Association of Nuclear Pore Fg-repeat Domains to Ntf2 Import and Export Complexes.. J. Mol. Biol. Feb 2007;366:330-45
Abstract
Transport into and out of the nucleus is regulated by the nuclear pore complex. Vital to this regulation are nuclear pore proteins with FG sequence repeats, which have been shown to be crucial for cell viability and which interact with nuclear transport receptors. Here we use molecular dynamics simulations to investigate the binding of FG-repeat peptides to the surface of NTF2, the Ran importer. The simulations, covering over 254 ns, agree with previous X-ray, mutational, NMR, and computational data in identifying four binding spots. They also serve to provide an all-atom view of binding at each spot, whereas FG-repeat binding has been only directly observed at a single spot. Furthermore, the simulations identify two novel binding spots in addition to the four others. All six binding spots broadly form a stripe across the surface of NTF2. The resulting regularity and proximity of binding spots on the surface may be necessary for identification of the transport receptor by the FG-repeats in the nuclear pore complex and for the successful transit of NTF2 through the pore.
Mesh Headings (Keywords): Active Transport, Cell Nucleus, Amino Acid Sequence, Binding Sites, Computer Simulation, Molecular Sequence Data, Nuclear Pore, Nucleocytoplasmic Transport Proteins, Protein Structure, Tertiary, Solutions, Structure-Activity Relationship
Check for Full Text / PubMed Unique Identifier (PMID): 17161424
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.