A Fail-safe System for the Ribosome Under Zinc-limiting Conditions in Bacillus Subtilis.
From: Laboratory of Molecular Genetics and Research Information Center for Extremophile, College of Science, Rikkyo University, Toshima-ku Nishi-ikebukuro 3-34-1, Tokyo 171-8501, Japan.
Molecular microbiology
- Publish Date: Jan 2007
- ISSN: 0950-382X
- Volume: 63
- Issue: 1
- Pages: 294-307
- Medium: Print
- Language: English
- Citation (JAMA): Natori Yousuke, Nanamiya Hideaki, Akanuma Genki, et al. A Fail-safe System for the Ribosome Under Zinc-limiting Conditions in Bacillus Subtilis.. Mol. Microbiol. Jan 2007;63:294-307
Abstract
As zinc is an essential trace metal ion for all living cells, cells elaborate a variety of strategies to cope with zinc starvation. In Bacillus subtilis, genes encoding ribosomal proteins L31 and S14 are duplicated into two types: one type contains a zinc-binding motif (RpmE or RpsN), whereas the other does not (YtiA or YhzA). We have previously shown that displacement of RpmE (L31) by YtiA from already assembled ribosomes is controlled by zinc, and this replacement could contribute to zinc mobilization under zinc-limiting conditions. We propose here that the switch between the two types of S14 has a different significance. rpsN is indispensable for growth and depletion of RpsN results in defective 30S subunits. YhzA can functionally replace RpsN to allow continued ribosome assembly under zinc-limiting conditions. Unlike YtiA, YhzA appeared in the ribosome at a slower rate consistent with incorporation into newly synthesized, rather than pre-existing ribosomes. These results raise the possibility that YhzA is involved in a fail-safe system for the de novo synthesis of ribosomes under zinc-limiting conditions.
Mesh Headings (Keywords): Bacillus subtilis, Binding Sites, DNA-Binding Proteins, Gene Expression Regulation, Bacterial, Ribosomal Proteins, Ribosomes, Zinc
Check for Full Text / PubMed Unique Identifier (PMID): 17163968
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