Structural Mechanism and Photoprotective Function of Water-soluble Chlorophyll-binding Protein.
From: Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan.
The Journal of biological chemistry
- Publish Date: Mar 2007
- ISSN: 0021-9258
- Volume: 282
- Issue: 9
- Pages: 6525-31
- Medium: Print
- Language: English
- Citation (JAMA): Horigome Daisuke, Satoh Hiroyuki, Itoh Nobue, et al. Structural Mechanism and Photoprotective Function of Water-soluble Chlorophyll-binding Protein.. J. Biol. Chem. Mar 2007;282:6525-31
Abstract
A water-soluble chlorophyll-binding protein (WSCP) is the single known instance of a putative chlorophyll (Chl) carrier in green plants. Recently the photoprotective function of WSCP has been demonstrated by EPR measurements; the light-induced singlet-oxygen formation of Chl in the WSCP tetramer is about four times lower than that of unbound Chl. This paper describes the crystal structure of the WSCP-Chl complex purified from leaves of Lepidium virginicum (Virginia pepperweed) to clarify the mechanism of its photoprotective function. The WSCP-Chl complex is a homotetramer comprising four protein chains of 180 amino acids and four Chl molecules. At the center of the complex one hydrophobic cavity is formed in which all of the four Chl molecules are tightly packed and isolated from bulk solvent. With reference to the novel Chl-binding mode, we propose that the photoprotection mechanism may be based on the inhibition of physical contact between the Chl molecules and molecular oxygen.
Mesh Headings (Keywords): Binding Sites, Chlorophyll, Crystallography, X-Ray, Lepidium, Light-Harvesting Protein Complexes, Plant Proteins, Protein Conformation
Check for Full Text / PubMed Unique Identifier (PMID): 17170107
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.