Mibefradil-sensitive Component Involved in the Plateau Potential in Submucosal Interstitial Cells of the Murine Proximal Colon.
From: Department of Regulatory Cell Physiology, Graduate School of Medical Sciences, Nagoya City University, Nagoya 467-8601, Japan. ayahotta@med.nagoya-cu.ac.jp
Biochemical and biophysical research communications
- Publish Date: Feb 2007
- ISSN: 0006-291X
- Volume: 353
- Issue: 1
- Pages: 170-6
- Medium: Print
- Language: English
- Citation (JAMA): Hotta Aya, Okada Noriko, Suzuki Hikaru, et al. Mibefradil-sensitive Component Involved in the Plateau Potential in Submucosal Interstitial Cells of the Murine Proximal Colon.. Biochem. Biophys. Res. Commun. Feb 2007;353:170-6
Abstract
Submucosal interstitial cells of Cajal (ICC(SM)) produce plateau potentials comprised of initial fast and subsequent plateau components. The possible involvement of voltage-dependent Ca(2+) channels in plateau potentials was examined in ICC(SM) of the murine proximal colon. Increases in external K(+) concentration ([K(+)](o)) changed the rise rate of the initial component in a biphasic way, an increase in 10.6 or 15.3mM [K(+)](o) and a decrease in 20.0mM [K(+)](o). The rise rate of plateau potentials was significantly reduced by the application of 3 microM mibefradil or 100 microM Ni(2+) but not by 0.3 microM nifedipine. The inhibitory effect of mibefradil on the rise rate of plateau potentials was concentration-dependent with an IC(50) value of 1.0 microM. In conclusion, the initial phase of plateau potentials is partly due to the activation of T-type Ca(2+) channel in ICC(SM) from the murine proximal colon.
Mesh Headings (Keywords): Action Potentials, Animals, Biological Clocks, Calcium Channel Blockers, Cells, Cultured, Colon, Dose-Response Relationship, Drug, Intestinal Mucosa, Male, Membrane Potentials, Mibefradil, Mice, Mice, Inbred BALB C, Submucous Plexus
Check for Full Text / PubMed Unique Identifier (PMID): 17174936
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