Interaction of Rice Dwarf Virus Outer Capsid P8 Protein with Rice Glycolate Oxidase Mediates Relocalization of P8.
From: Peking-Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology, The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing, PR China.
FEBS letters
- Publish Date: Jan 2007
- ISSN: 0014-5793
- Volume: 581
- Issue: 1
- Pages: 34-40
- Medium: Print
- Language: English
- Citation (JAMA): Zhou Feng, Wu Gang, Deng Wulan, et al. Interaction of Rice Dwarf Virus Outer Capsid P8 Protein with Rice Glycolate Oxidase Mediates Relocalization of P8.. FEBS Lett. Jan 2007;581:34-40
Abstract
Yeast two-hybrid and coimmunoprecipitation assays indicated that P8, an outer capsid protein of Rice dwarf phytoreovirus (RDV), interacts with rice glycolate oxidase (GOX), a typical enzyme of peroxisomes. Confocal immunofluorescence microscopy revealed that P8 was colocalized with GOX in peroxisomes. Time course analysis demonstrated that the localization of P8 in Spodoptera frugiperda cells changed from diffuse to discrete, punctuate inclusions during expression from 24 to 48 h post inoculation. Coexpression of GOX with P8 may target P8 into peroxisomes, which serve as replication sites for a number of viruses. Therefore, we conclude that the interaction of P8 with the GOX of host cells leads to translocation of P8 into peroxisomes and we further propose that the interaction between P8 and GOX may play important roles in RDV targeting into the replication site of host cells. Our findings have broad significance in studying the mechanisms whereby viruses target appropriate replication sites and begin their replication.
Mesh Headings (Keywords): Alcohol Oxidoreductases, Animals, Capsid Proteins, Cell Line, Oryza sativa, Peroxisomes, Plant Proteins, Protein Transport, Reoviridae, Spodoptera, Virus Replication
Check for Full Text / PubMed Unique Identifier (PMID): 17174956
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.