Mechanism of Allosteric Regulation of Transglutaminase 2 by Gtp.
From: Victor Chang Cardiac Research Institute, University of New South Wales, 384 Victoria Street, Darlinghurst NSW 2010, Australia.
Proceedings of the National Academy of Sciences of the United States of America
- Publish Date: Dec 2006
- ISSN: 0027-8424
- Volume: 103
- Issue: 52
- Pages: 19683-8
- Medium: Print
- Language: English
- Citation (JAMA): Begg Gillian E, Carrington Lyle, Stokes Philippa H, et al. Mechanism of Allosteric Regulation of Transglutaminase 2 by Gtp.. Proc. Natl. Acad. Sci. U.S.A. Dec 2006;103:19683-8
Abstract
Allosteric regulation is a fundamental mechanism of biological control. Here, we investigated the allosteric mechanism by which GTP inhibits cross-linking activity of transglutaminase 2 (TG2), a multifunctional protein, with postulated roles in receptor signaling, extracellular matrix assembly, and apoptosis. Our findings indicate that at least two components are involved in functionally coupling the allosteric site and active center of TG2, namely (i) GTP binding to mask a conformationally destabilizing switch residue, Arg-579, and to facilitate interdomain interactions that promote adoption of a compact, catalytically inactive conformation and (ii) stabilization of the inactive conformation by an uncommon H bond between a cysteine (Cys-277, an active center residue) and a tyrosine (Tyr-516, a residue located on a loop of the beta-barrel 1 domain that harbors the GTP-binding site). Although not essential for GTP-mediated inhibition of cross-linking, this H bond enhances the rate of formation of the inactive conformer.
Mesh Headings (Keywords): Allosteric Regulation, Animals, Arginine, Binding Sites, Cysteine, Disulfides, GTP-Binding Proteins, Guanosine Triphosphate, Hydrogen Bonding, Models, Molecular, Mutation, Protein Binding, Protein Structure, Tertiary, Rats, Transglutaminases, Tyrosine, Water
Check for Full Text / PubMed Unique Identifier (PMID): 17179049
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