Identification of Proteins Whose Expression is Up- or Down-regulated in the Mushroom Bodies in the Honeybee Brain Using Proteomics.
From: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan.
FEBS letters
- Publish Date: Jan 2007
- ISSN: 0014-5793
- Volume: 581
- Issue: 1
- Pages: 97-101
- Medium: Print
- Language: English
- Citation (JAMA): Uno Yuko, Fujiyuki Tomoko, Morioka Mizue, et al. Identification of Proteins Whose Expression is Up- or Down-regulated in the Mushroom Bodies in the Honeybee Brain Using Proteomics.. FEBS Lett. Jan 2007;581:97-101
Abstract
To identify protein(s) with different expression patterns in the mushroom bodies (MBs) in the honeybee brain, we compared the protein profiles of MBs and optic lobes (OLs) using proteomics. Two-dimensional gel electrophoresis revealed that five and three spots were selectively expressed in the MBs or OLs, respectively. Liquid chromatography tandem mass spectrometry analysis identified juvenile hormone diol kinase and glyceraldehyde-3-phosphate dehydrogenase as MB- and OL-selective proteins, respectively. In situ hybridization revealed that jhdk expression was upregulated in MB neuron subsets, whereas gapdh expression was downregulated, indicating that MBs have a distinct gene and protein expression profile in the honeybee brain.
Mesh Headings (Keywords): Animals, Bees, Brain Chemistry, Down-Regulation, Electrophoresis, Gel, Two-Dimensional, In Situ Hybridization, Insect Proteins, Mushroom Bodies, Proteome, Proteomics, Up-Regulation
Check for Full Text / PubMed Unique Identifier (PMID): 17182037
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